Synthesis of prostaglandin E1 phosphate derivatives and their encapsulation in biodegradable nanoparticles

Miho Takeda, Taishi Maeda, Tsutomu Ishihara, Haruka Sakamoto, Kanae Yuki, Naoko Takasaki, Fumihiro Nishimura, Takeshi Yamashita, Ken Ichiro Tanaka, Mitsuko Takenaga, Rie Igarashi, Megumu Higaki, Naoki Yamakawa, Yoshinari Okamoto, Hisao Ogawa, Masami Otsuka, Yutaka Mizushima, Tohru Mizushima

研究成果: Article査読

8 被引用数 (Scopus)

抄録

Purpose: Prostaglandin E1 (PGE1) is an effective treatment for peripheral vascular diseases. The encapsulation of PGE1 in nanoparticles for its sustained-release would improve its therapeutic effect and quality of life (QOL) of patients. Methods: In order to encapsulate PGE1 in nanoparticles prepared with a poly(lactide) homopolymer (PLA) and monomethoxy poly(ethyleneglycol)-PLA block copolymer (PEG-PLA), we synthesized a series of PGE1 phosphate derivatives and tested their efficacy. Results: Among them, PGE1 2-(phosphonooxy)ethyl ester sodium salt (C2) showed the most efficient hydrolysis to yield PGE1 in human serum. An in vitro platelet aggregation assay showed that C2 inhibited aggregation only after pre-incubation in serum, suggesting that C2 is a prodrug of PGE1. In vivo, intravenous administration of C2 caused increase in cutaneous blood flow. In the presence of zinc ions, all of the synthesized PGE1 phosphate derivatives could be encapsulated in PLA-nanoparticles. Use of l-PLA instead of d,l-PLA, and high molecular weight PLA resulted in a slower release of C2 from the nanoparticles. Conclusions: We consider that C2-encapsulated nanoparticles prepared with l-PLA and PEG-d,l-PLA have good sustained-release profile of PGE1, which is useful clinically.

本文言語English
ページ(範囲)1792-1800
ページ数9
ジャーナルPharmaceutical research
26
7
DOI
出版ステータスPublished - 2009 7

ASJC Scopus subject areas

  • バイオテクノロジー
  • 分子医療
  • 薬理学
  • 薬科学
  • 有機化学
  • 薬理学(医学)

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