Systematic repression of transcription factors reveals limited patterns of gene expression changes in ES cells

Akira Nishiyama, Alexei A. Sharov, Yulan Piao, Misa Amano, Tomokazu Amano, Hien G. Hoang, Bernard Y. Binder, Richard Tapnio, Uwem Bassey, Justin N. Malinou, Lina S. Correa-Cerro, Hong Yu, Li Xin, Emily Meyers, Michal Zalzman, Yuhki Nakatake, Carole Stagg, Lioudmila Sharova, Yong Qian, Dawood DudekulaSarah Sheer, Jean S. Cadet, Tetsuya Hirata, Hsih Te Yang, Ilya Goldberg, Michele K. Evans, Dan L. Longo, David Schlessinger, Minoru S.H. Ko

研究成果: Article査読

39 被引用数 (Scopus)

抄録

Networks of transcription factors (TFs) are thought to determine and maintain the identity of cells. Here we systematically repressed each of 100 TFs with shRNA and carried out global gene expression profiling in mouse embryonic stem (ES) cells. Unexpectedly, only the repression of a handful of TFs significantly affected transcriptomes, which changed in two directions/ trajectories: one trajectory by the repression of either Pou5f1 or Sox2; the other trajectory by the repression of either Esrrb, Sall4, Nanog, or Tcfap4. The data suggest that the trajectories of gene expression change are already preconfigured by the gene regulatory network and roughly correspond to extraembryonic and embryonic fates of cell differentiation, respectively. These data also indicate the robustness of the pluripotency gene network, as the transient repression of most TFs did not alter the transcriptomes.

本文言語English
論文番号1390
ジャーナルScientific reports
3
DOI
出版ステータスPublished - 2013 3 25

ASJC Scopus subject areas

  • General

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