Tagging of avidin immobilized beads with biotinylated YAG:Ce3+ nanocrystal phosphor

Ryo Asakura, Tetsuhiko Isobe, Kiyoshi Kurokawa, Hideki Aizawa, Michio Ohkubo

研究成果: Article査読

40 被引用数 (Scopus)

抄録

YAG:Ce3+ nanoparticles 9.5∈±∈1.2 nm in diameter have been synthesized from aluminium isopropoxide and acetates of yttrium and cerium in 1,4-butanediol (1,4-BD) by autoclave treatment at 300 °C for 2 h. After replacing 1,4-BD by ultrapure water, NH2 groups were introduced on the surface of YAG:Ce3+ nanoparticles by addition of 3-aminopropyltrimethoxysilane then biotinylation with sulfo-NHS-LC-biotin. We demonstrated that avidin immobilized beads are tagged by biotinylated YAG:Ce3+ nanoparticles by the selective avidin-biotin interaction, furnishing a green fluorescent image on excitation with blue light. This result indicates that YAG:Ce3+ nanoparticle phosphors have much potential in biological applications.

本文言語English
ページ(範囲)1641-1647
ページ数7
ジャーナルAnalytical and bioanalytical chemistry
386
6
DOI
出版ステータスPublished - 2006 11 1

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry

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