Talbot-defocus multiscan tomography using the synchrotron X-ray microscope to study the lacuno-canalicular network in mouse bone

Nobuhito Nango, Shogo Kubota, Akihisa Takeuchi, Yoshio Suzuki, Wataru Yashiro, Atsushi Momose, Koichi Matsuo

研究成果: Article査読

11 被引用数 (Scopus)

抄録

The three-dimensional network of lacunae and canaliculi that regulates metabolism in bone contains osteocytes and their dendritic processes. We constructed a synchrotron radiation X-ray microscope for sequential tomography of mouse tibia first by using a Talbot interferometer to detect the degree of bone mineralization and then by using absorption contrast under a slightly defocused setting to enhance outline contrast thereby visualizing structures of the osteocyte lacuno-canalicular network. The resultant pair of tomograms was precisely aligned with each other, allowing evaluation of mineral density in the vicinity of each osteocyte lacuna and canaliculus over the entire thickness of the cortical bone. Thus, multiscan microscopic X-ray tomography is a powerful tool for analyzing bone mineralization in relation to the lacuno-canalicular network at the submicron resolution level.

本文言語English
ページ(範囲)917-923
ページ数7
ジャーナルBiomedical Optics Express
4
6
DOI
出版ステータスPublished - 2013

ASJC Scopus subject areas

  • バイオテクノロジー
  • 原子分子物理学および光学

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