TAP/NXF1, the primary mRNA export receptor, specifically interacts with a neuronal RNA-binding protein HuD

Kuniaki Saito, Toshinobu Fujiwara, Jun Katahira, Kunio Inoue, Hiroshi Sakamoto

研究成果: Article査読

24 被引用数 (Scopus)

抄録

Hu proteins are RNA-binding proteins that are implicated in the control of stabilization, nuclear export, and/or translation of specific mRNAs with AU-rich elements (AREs) in the 3′-untranslated region. Three neuron-specific Hu proteins (HuD, HuB, and HuC), but not a ubiquitously expressed Hu protein HuR, have an activity to induce neurite outgrowth when they are overexpressed in a rat neuronal cell line PC12. Here we show that TAP/NXF1, the primary export receptor for the bulk mRNA, is a specific binding partner for HuD. In vitro binding experiments using recombinant proteins revealed that the interaction between TAP and HuD is direct and that HuD can form a ternary complex together with both TAP and RNA. Interestingly, HuR does not interact with TAP. These results suggest that HuD acts as a novel adaptor protein to recruit TAP for efficient export of ARE-containing mRNAs in neuronal cells.

本文言語English
ページ(範囲)291-297
ページ数7
ジャーナルBiochemical and Biophysical Research Communications
321
2
DOI
出版ステータスPublished - 2004 8月 20

ASJC Scopus subject areas

  • 生物理学
  • 生化学
  • 分子生物学
  • 細胞生物学

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