Targeting of liposomes carrying recombinant fragments of platelet membrane glycoprotein Ibα to immobilized von Willebrand factor under flow conditions

Takako Nishiya, Mitsuru Murata, Makoto Handa, Yasuo Ikeda

研究成果: Article

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Liposomes with covalently bound recombinant fragments of platelet membrane glycoprotein Ibα that retain the von Willebrand factor (vWf)-binding function (rGPIbα-liposomes) were prepared. Their interactions with an immobilized vWf surface under flow conditions were evaluated with a recirculating flow chamber, mounted on an epifluorescence microscope, which allows real-time visualization of fluorescence-labeled liposomes interacting with the surface. The interaction of rGPIbα-liposomes with the vWf surface was directly related to shear rate. At high densities of rGPIbα and vWf, rGPIbα-liposomes establishing contact with the vWf surface exhibited continuous displacement with decreased velocity relative to the hydrodynamic flow, depending on receptor density and matrix concentration. At lower densities of rGPIbα and vWf, rGPIbα-liposomes stopped only transiently, in the millisecond range, on the surface. This is the first study to demonstrate that the targeting of rGPIbα-liposomes is specific to the vWf surface under flow conditions. (C) 2000 Academic Press.

元の言語English
ページ(範囲)755-760
ページ数6
ジャーナルBiochemical and Biophysical Research Communications
270
発行部数3
DOI
出版物ステータスPublished - 2000 4 21

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ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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