TCR sequencing analysis of cancer tissues and tumor draining lymph nodes in colorectal cancer patients

Tatsuo Matsuda, Eisaku Miyauchi, Yu Wen Hsu, Satoshi Nagayama, Kazuma Kiyotani, Makda Zewde, Jae Hyun Park, Taigo Kato, Makiko Harada, Shimpei Matsui, Masashi Ueno, Kazumasa Fukuda, Nobuaki Suzuki, Shoichi Hazama, Hiroaki Nagano, Hiroya Takeuchi, Wickii T. Vigneswaran, Yuko Kitagawa, Yusuke Nakamura

研究成果: Article査読

9 被引用数 (Scopus)

抄録

Tumor draining lymph nodes (TDLNs) are located in the routes of lymphatic drainage from a primary tumor and have the highest risk of metastasis in various types of solid tumors. TDLNs are also considered as a tissue to activate the antitumor immunity, where antigen-specific effector T cells are generated. However, T cell receptor (TCR) repertoires in TDLNs have not been well characterized. We collected 23 colorectal cancer tumors with 203 lymph nodes with/without metastatic cancer cells (67 were metastasis-positive and the remaining 136 were metastasis-negative) and performed TCR sequencing. Metastasis-positive TDLNs showed a significantly lower TCR diversity and shared TCR clonotypes more frequently with primary tumor tissues compared to metastasis-negative TDLNs. Principal component analysis indicated that TDLNs with metastasis showed similar TCR repertoires. These findings suggest that cancer-reactive T cell clones could be enriched in the metastasis-positive TDLNs.

本文言語English
論文番号e1588085
ジャーナルOncoImmunology
8
6
DOI
出版ステータスPublished - 2019 6 3

ASJC Scopus subject areas

  • 免疫アレルギー学
  • 免疫学
  • 腫瘍学

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