Technical feasibility of visualizing myenteric plexus using confocal laser endomicroscopy

Masakuni Kobayashi, Kazuki Sumiyama, Naoki Shimojima, Satoshi Ieiri, Hideyuki Okano, Shunsuke Kamba, Takumi Fujimura, Seiichi Hirobe, Tatsuo Kuroda, Junko Takahashi-Fujigasaki

研究成果: Article

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Background and Aim: In preceding studies, we identified that the myenteric plexus (MP) could be visualized with confocal laser endomicroscopy (CLE) by applying neural fluorescent probes lacking clinical safety profiling data from the submucosal side. In this study, we evaluated the technical feasibility of MP visualization using probe-based CLE (pCLE) from the serosal side with cresyl violet (CV), which has been used clinically for chromoendoscopy. Methods: The dye affinity of CV for MP was first explored in an in vivo transgenic mouse model using neural crest derivatives labeled with green fluorescent protein. We also tested the feasibility of CV-assisted visualization of MP in human surgical specimens, wherein the tissue dying and pCLE observation were performed from the serosal side. In the human study, rate of MP visualization by pCLE was evaluated as the primary outcome. We also evaluated the sensitivity and specificity of MP visualization by pCLE, using pathological presence/absence of MP as the gold standard. Results: We confirmed the dye affinity of CV to MP in all tested models. The MP appeared as brightly stained ladder-like structures with pCLE, and in the human study, MP was visualized in 12/14 (85.7%) samples, with 92.3% sensitivity and 100% specificity. In positive cases showing the ladder-like structure of MP by pCLE, the mean maximum and minimum widths of nerve strands were 54.3 (± 23.6) and 19.7 (± 6.0) μm, respectively. A ganglion was detected by pCLE in 10 cases (10/12, 83.3%). Conclusions: This study demonstrated the technical feasibility of visualizing the MP in real time by CV-assisted pCLE (UMIN-CTR number, UMIN000015056).

元の言語English
ページ(範囲)1604-1610
ページ数7
ジャーナルJournal of Gastroenterology and Hepatology (Australia)
32
発行部数9
DOI
出版物ステータスPublished - 2017 9 1

ASJC Scopus subject areas

  • Hepatology
  • Gastroenterology

フィンガープリント Technical feasibility of visualizing myenteric plexus using confocal laser endomicroscopy' の研究トピックを掘り下げます。これらはともに一意のフィンガープリントを構成します。

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    Kobayashi, M., Sumiyama, K., Shimojima, N., Ieiri, S., Okano, H., Kamba, S., Fujimura, T., Hirobe, S., Kuroda, T., & Takahashi-Fujigasaki, J. (2017). Technical feasibility of visualizing myenteric plexus using confocal laser endomicroscopy. Journal of Gastroenterology and Hepatology (Australia), 32(9), 1604-1610. https://doi.org/10.1111/jgh.13754