Temperature-Responsive Chromatography Using Poly-(N-isopropylacrylamide) Hydrogel-Modified Silica

Hideko Kanazawa, Tatsuo Sunamoto, Eri Ayano, Yoshikazu Matsushima, Akihiko Kikuchi, Teruo Okano

研究成果: Article査読

78 被引用数 (Scopus)

抄録

Poly(N-isopropylacrylamide) (PNIPAAm) has the sharpest phase transition of the class of thermo-sensitive N-alkyl acrylamide polymers. We developed a new method of HPLC using packing materials modified with cross-linked poly(N-isopropylacrylamide) (PNIPAAm) hydrogel. A temperature-responsive surface was prepared by polymerization of NIPAAm in the presence of a cross-linker on the silica support. The surface properties and functions of the stationary phases change in response to the external temperature. Therefore it easily changes the interaction of a solute with the surface with a constant aqueous mobile phase. A temperature-responsive elution behavior was observed on the separation of steroids and PTH-amino acids. The method is expected to be applicable to separation in the pharmaceutical and biomedical fields.

本文言語English
ページ(範囲)45-48
ページ数4
ジャーナルanalytical sciences
18
1
DOI
出版ステータスPublished - 2002

ASJC Scopus subject areas

  • 分析化学

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