Temperature-responsive chromatography using poly(N-isopropylacrylamide)-modified silica

Hideko Kanazawa, Kazuo Yamamoto, Yoshikazu Matsushima, Nobuharu Takai, Akihiko Kikuchi, Yasuhisa Sakurai, Teruo Okano

研究成果: Article査読

401 被引用数 (Scopus)

抄録

A new concept in chromatography is proposed that utilizes a temperature-responsive surface with a constant aqueous mobile phase. The surface of the silica stationary phase in high-performance liquid chromatography (HPLC) has been modified with temperature-responsive polymers to exhibit temperature-controlled hydrophilic/hydrophobic changes. Poly(N-isopropylacrylamide) (PEPAAm) was grafted onto (aminopropyl)silica using an activated ester- amine coupling method. These grafted silica surfaces show hydrophilic properties at lower temperatures which, as temperature increases, transform to hydrophobic surface properties. The elution profile of five mixed steroids on an HPLC column packed with this material depends largely on the temperature of the aqueous mobile phase. Retention times increase with increasing temperature without any change in the eluent. Changes in the retention times of hydrophobic steroids were larger man those for hydrophilic steroids. The temperature-responsive interaction between PDPAAm-modified silica and these steroids is proposed to result from changes in the surface properties of the HPLC stationary phase by the transition of hydrophilic/hydrophobic surface-grafted IPAAm polymers. We demonstrate a novel and useful new chromatography system hi which surface properties and the resulting function of the HPLC stationary phase are controlled by external temperature changes. This method should be effective in biological and biomedical separations of peptides and proteins using only aqueous mobile phases.

本文言語English
ページ(範囲)100-105
ページ数6
ジャーナルAnalytical chemistry
68
1
出版ステータスPublished - 1996 12月 1

ASJC Scopus subject areas

  • 分析化学

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