Temperature-responsive solid-phase extraction column for biological sample pretreatment

Michiko Akimaru, Kohei Okubo, Yuki Hiruta, Hideko Kanazawa

研究成果: Article査読

17 被引用数 (Scopus)

抄録

We have developed a novel solid-phase extraction (SPE) system utilizing a temperature-responsive polymer hydrogelmodified stationary phase. Aminopropyl silica beads (average diameter, 40 - 64 μm) were coated with poly(N-isopropylacrylamide) (PNIPAAm)-based thermo-responsive hydrogels. Butyl methacrylate (BMA) and N,N-dimethylaminopropyl acrylamide (DMAPAAm) were used as the hydrophobic and cationic monomers, respectively, and copolymerized with NIPAAm. To evaluate the use of this SPE cartridge for the analysis of drugs and proteins in biological fluids, we studied the separation of phenytoin and theophylline from human serum albumin (HSA) as a model system. The retention of the analytes in an exclusively aqueous eluent could be modulated by changing the temperature and salt content. These results indicated that this temperature-responsive SPE system can be applied to the pretreatment of biological samples for the measurement of serum drug levels. 2015

本文言語English
ページ(範囲)881-886
ページ数6
ジャーナルanalytical sciences
31
9
DOI
出版ステータスPublished - 2015

ASJC Scopus subject areas

  • 分析化学

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