Temporal focusing microscopy combined with three-dimensional structured illumination

Keisuke Isobe, Keisuke Toda, Qiyuan Song, Fumihiko Kannari, Hiroyuki Kawano, Atsushi Miyawaki, Katsumi Midorikawa

研究成果: Article

3 引用 (Scopus)

抄録

Temporal focusing microscopy provides the optical sectioning capability in wide-field two-photon fluorescence imaging. Here, we demonstrate temporal focusing microscopy combined with three-dimensional structured illumination, which enables us to enhance the three-dimensional spatial resolution and reject the background fluorescence. Experimentally, the periodic pattern of the illumination was produced not only in the lateral direction but also in the axial direction by the interference between three temporal focusing pulses, which were easily generated using a digital micromirror device. The lateral resolution and optical sectioning capability were successfully enhanced by factors of 1.6 and 3.6, respectively, compared with those of temporal focusing microscopy. In the two-photon fluorescence imaging of a tissue-like phantom, the out-of-focus background fluorescence and the scattered background fluorescence could also be rejected.

元の言語English
記事番号052501
ジャーナルJapanese Journal of Applied Physics
56
発行部数5
DOI
出版物ステータスPublished - 2017 5 1

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Microscopic examination
Lighting
Fluorescence
illumination
microscopy
fluorescence
Photons
Imaging techniques
Digital devices
photons
spatial resolution
Tissue
interference
pulses

ASJC Scopus subject areas

  • Engineering(all)
  • Physics and Astronomy(all)

これを引用

Temporal focusing microscopy combined with three-dimensional structured illumination. / Isobe, Keisuke; Toda, Keisuke; Song, Qiyuan; Kannari, Fumihiko; Kawano, Hiroyuki; Miyawaki, Atsushi; Midorikawa, Katsumi.

:: Japanese Journal of Applied Physics, 巻 56, 番号 5, 052501, 01.05.2017.

研究成果: Article

Isobe, Keisuke ; Toda, Keisuke ; Song, Qiyuan ; Kannari, Fumihiko ; Kawano, Hiroyuki ; Miyawaki, Atsushi ; Midorikawa, Katsumi. / Temporal focusing microscopy combined with three-dimensional structured illumination. :: Japanese Journal of Applied Physics. 2017 ; 巻 56, 番号 5.
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