Temporal profile and cellular localization of interleukin-6 protein after focal cerebral ischemia in rats

Shigeaki Suzuki, Kortaro Tanaka, Shigeru Nogawa, Eiichiro Nagata, Daisuke Ito, Tomohisa Dembo, Yasuo Fukuuchi

研究成果: Article

97 引用 (Scopus)

抄録

Although interleukin-6 (IL-6) has various neuroprotective effects against cerebral ischemia, the topographic distribution and cellular source of IL-6 after cerebral ischemia remain unclear. In the current study, the localization of IL-6 protein was immunohistochemically examined in rats after 3.5, 12, 24, and 48 hours of reperfusion after 1.5 hours of middle cerebral artery occlusion. Middle cerebral artery occlusion was induced by the intraluminal suture method. The specificity of the anti-IL-6 antibody used in the current study was confirmed by Western blot analysis and an immunoabsorption text. To identify tile cellular source, lectin histochemical study and immunohistochemical study with microtubule-associated protein-2, ED1 and glial fibrillary acidic protein also were carried out. The sham group did not show any clear IL-6 immunoreactivity. After 3.5 hours of reperfusion, IL-6 immunoreactivity was first detected on tile reperfused side, and it was upregulated, especially in the periinfarct region, after 24 hours of reperfusion. Also, IL-6 was expressed after 3.5 hours of reperfusion in the contralateral cerebral cortex and bilateral hippocampi. Double staining showed that the cells containing IL-6 were neurons and round-type microglia, not astrocytes. The current findings suggest that IL-6 expression in ischemically threatened neurons and reactive microglia is closely associated with brain tissue neuroprotective mechanisms against cerebral ischemia.

元の言語English
ページ(範囲)1256-1262
ページ数7
ジャーナルJournal of Cerebral Blood Flow and Metabolism
19
発行部数11
出版物ステータスPublished - 1999

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Brain Ischemia
Interleukin-6
Proteins
Reperfusion
Middle Cerebral Artery Infarction
Microglia
Neurons
Microtubule-Associated Proteins
Glial Fibrillary Acidic Protein
Neuroprotective Agents
Lectins
Astrocytes
Cerebral Cortex
Sutures
Hippocampus
Western Blotting
Staining and Labeling
Antibodies
Brain

ASJC Scopus subject areas

  • Endocrinology
  • Neuroscience(all)
  • Endocrinology, Diabetes and Metabolism

これを引用

Temporal profile and cellular localization of interleukin-6 protein after focal cerebral ischemia in rats. / Suzuki, Shigeaki; Tanaka, Kortaro; Nogawa, Shigeru; Nagata, Eiichiro; Ito, Daisuke; Dembo, Tomohisa; Fukuuchi, Yasuo.

:: Journal of Cerebral Blood Flow and Metabolism, 巻 19, 番号 11, 1999, p. 1256-1262.

研究成果: Article

Suzuki, Shigeaki ; Tanaka, Kortaro ; Nogawa, Shigeru ; Nagata, Eiichiro ; Ito, Daisuke ; Dembo, Tomohisa ; Fukuuchi, Yasuo. / Temporal profile and cellular localization of interleukin-6 protein after focal cerebral ischemia in rats. :: Journal of Cerebral Blood Flow and Metabolism. 1999 ; 巻 19, 番号 11. pp. 1256-1262.
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abstract = "Although interleukin-6 (IL-6) has various neuroprotective effects against cerebral ischemia, the topographic distribution and cellular source of IL-6 after cerebral ischemia remain unclear. In the current study, the localization of IL-6 protein was immunohistochemically examined in rats after 3.5, 12, 24, and 48 hours of reperfusion after 1.5 hours of middle cerebral artery occlusion. Middle cerebral artery occlusion was induced by the intraluminal suture method. The specificity of the anti-IL-6 antibody used in the current study was confirmed by Western blot analysis and an immunoabsorption text. To identify tile cellular source, lectin histochemical study and immunohistochemical study with microtubule-associated protein-2, ED1 and glial fibrillary acidic protein also were carried out. The sham group did not show any clear IL-6 immunoreactivity. After 3.5 hours of reperfusion, IL-6 immunoreactivity was first detected on tile reperfused side, and it was upregulated, especially in the periinfarct region, after 24 hours of reperfusion. Also, IL-6 was expressed after 3.5 hours of reperfusion in the contralateral cerebral cortex and bilateral hippocampi. Double staining showed that the cells containing IL-6 were neurons and round-type microglia, not astrocytes. The current findings suggest that IL-6 expression in ischemically threatened neurons and reactive microglia is closely associated with brain tissue neuroprotective mechanisms against cerebral ischemia.",
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AU - Tanaka, Kortaro

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AU - Nagata, Eiichiro

AU - Ito, Daisuke

AU - Dembo, Tomohisa

AU - Fukuuchi, Yasuo

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