Terminal differentiation of murine resident peritoneal macrophages is characterized by expression of the STK protein tyrosine kinase, a receptor for macrophage-stimulating protein

Atsushi Iwama, Ming Hai Wang, Naoto Yamaguchi, Noriko Ohno, Kiyoshi Okano, Tetsuo Sudo, Motohiro Takeya, Francine Gervais, Celine Morissette, Edward J. Leonard, Toshio Suda

研究成果: Article査読

94 被引用数 (Scopus)

抄録

STK, a new member of the hepatocyte growth factor receptor family, is the receptor for macrophage-stimulating protein (MSP), which acts on murine resident peritoneal macrophages. We established polyclonal and monoclonal antibodies against STK and characterized the structure of STK protein and STK expression on cells of the mononuclear phagocyte system. Western blotting showed that the STK transcript is translated into a single-chain precursor and then cleaved into a 165-kD disulfide-linked heterodimer composed of a 35- kD α-chain and a 144-kD β-chain. Western blotting detected STK protein on resident peritoneal macrophages, a target of MSP, and showed that it was autophosphorylated in cells stimulated by MSP. By flow cytometric analysis using a monoclonal anti-STK antibody, we showed that STK protein is expressed on restricted macrophage populations such as resident peritoneal macrophages, but not on exudate peritoneal macrophages or mononuclear phagocytes of the bone marrow, peripheral blood, spleen, or alveoli. Resident peritoneal macrophages were classified into two fractions according to their reactivity with an anti-STK antibody and a marker antibody for macrophages: STK(high)- F4/80(high) cells and STK(negative)-F4/80(low) cells. Acute exudative macrophages were all STK(negative)-F4/80(low), but they gradually became predominantly STK(high)-F4/80(low) several days after entrance into the peritoneal cavity. These results showed that after monocytes migrate into the peritoneal cavity, they undergo terminal differentiation in the peritoneal microenvironment. This is the first evidence of tissue-specific terminal differentiation of peritoneal macrophages, and this terminal differentiation can be characterized by the expression of STK receptor tyrosine kinase.

本文言語English
ページ(範囲)3394-3403
ページ数10
ジャーナルBlood
86
9
DOI
出版ステータスPublished - 1995 11 1

ASJC Scopus subject areas

  • 生化学
  • 免疫学
  • 血液学
  • 細胞生物学

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