Th17 cells carrying TCR recognizing epidermal autoantigen induce psoriasis-like skin inflammation

Shuhei Nishimoto, Hitoshi Kotani, Sanae Tsuruta, Nana Shimizu, Minako Ito, Takashi Shichita, Rimpei Morita, Hayato Takahashi, Masayuki Amagai, Akihiko Yoshimura

研究成果: Article

23 引用 (Scopus)

抄録

Psoriasis is considered a Th17-type autoimmune skin inflammatory disease; however, involvement of an autoantigen-specific TCR has not been established. In this study, we show that psoriasis-like skin inflammation can be induced by autoreactive Th17 cells.We previously developed the desmoglein 3-specific TCR-transgenic (Dsg3H1) mouse, in which CD4+ T cells recognize physiological epidermal autoantigen. T cells from Dsg3H1 mice were polarized into Th17 cells in vitro and then adoptively transferred into Rag2-/- mice. Dsg3H1-Th17 cells induced severe psoriasis-like skin inflammation within 2 wk after transfer in the tissues in which desmoglein 3 is expressed. Such pathology was not observed when wild-type Th17 cells or Th1-skewed Dsg3H1 T cells were transferred, and it was strongly suppressed by anti-IL-12/23 and anti-IL-17 Abs. Although IFN-γ+/IL-17+ T cells accumulated in the skin lesions of mice that received Dsg3H1-Th17 cells, IFN-γ-deficient Dsg3H1-Th17 cells were fully pathogenic. These results demonstrate that cutaneous psoriasis-like immunopathology can be developed by epidermis-specific recognition of Th17 cells, which is strictly dependent on IL-17 but not IFN-γ.

元の言語English
ページ(範囲)3065-3072
ページ数8
ジャーナルJournal of Immunology
191
発行部数6
DOI
出版物ステータスPublished - 2013 9 15

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Th17 Cells
Autoantigens
Psoriasis
Inflammation
Skin
Interleukin-17
Desmoglein 3
T-Lymphocytes
Interleukin-23
Interleukin-12
Skin Diseases
Epidermis
Transgenic Mice
Pathology

ASJC Scopus subject areas

  • Immunology

これを引用

Th17 cells carrying TCR recognizing epidermal autoantigen induce psoriasis-like skin inflammation. / Nishimoto, Shuhei; Kotani, Hitoshi; Tsuruta, Sanae; Shimizu, Nana; Ito, Minako; Shichita, Takashi; Morita, Rimpei; Takahashi, Hayato; Amagai, Masayuki; Yoshimura, Akihiko.

:: Journal of Immunology, 巻 191, 番号 6, 15.09.2013, p. 3065-3072.

研究成果: Article

Nishimoto, Shuhei ; Kotani, Hitoshi ; Tsuruta, Sanae ; Shimizu, Nana ; Ito, Minako ; Shichita, Takashi ; Morita, Rimpei ; Takahashi, Hayato ; Amagai, Masayuki ; Yoshimura, Akihiko. / Th17 cells carrying TCR recognizing epidermal autoantigen induce psoriasis-like skin inflammation. :: Journal of Immunology. 2013 ; 巻 191, 番号 6. pp. 3065-3072.
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AU - Shichita, Takashi

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