The APC/C activator Cdh1 regulates the G2/M transition during differentiation of placental trophoblast stem cells

Hideaki Naoe, Tatsuyuki Chiyoda, Jo Ishizawa, Kenta Masuda, Hideyuki Saya, Shinji Kuninaka

研究成果: Article

6 引用 (Scopus)

抄録

Differentiation of placental trophoblast stem (TS) cells to trophoblast giant (TG) cells is accompanied by transition from a mitotic cell cycle to an endocycle. Here, we report that Cdh1, a regulator of the anaphase-promoting complex/cyclosome (APC/C), negatively regulates mitotic entry upon the mitotic/endocycle transition. TS cells derived from homozygous Cdh1 gene-trapped (Cdh1GT/GT) murine embryos accumulated mitotic cyclins and precociously entered mitosis after induction of TS cell differentiation, indicating that Cdh1 is required for the switch from mitosis to the endocycle. Furthermore, the Cdh1GT/GT TS cells and placenta showed aberrant expression of placental differentiation markers. These data highlight an important role of Cdh1 in the G2/M transition during placental differentiation.

元の言語English
ページ(範囲)757-762
ページ数6
ジャーナルBiochemical and Biophysical Research Communications
430
発行部数2
DOI
出版物ステータスPublished - 2013 1 11

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Anaphase-Promoting Complex-Cyclosome
Trophoblasts
Stem cells
Stem Cells
Mitosis
Cyclins
Differentiation Antigens
Giant Cells
Genes
Placenta
Cells
Switches
Cell Differentiation
Cell Cycle
Embryonic Structures

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Cell Biology
  • Molecular Biology

これを引用

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abstract = "Differentiation of placental trophoblast stem (TS) cells to trophoblast giant (TG) cells is accompanied by transition from a mitotic cell cycle to an endocycle. Here, we report that Cdh1, a regulator of the anaphase-promoting complex/cyclosome (APC/C), negatively regulates mitotic entry upon the mitotic/endocycle transition. TS cells derived from homozygous Cdh1 gene-trapped (Cdh1GT/GT) murine embryos accumulated mitotic cyclins and precociously entered mitosis after induction of TS cell differentiation, indicating that Cdh1 is required for the switch from mitosis to the endocycle. Furthermore, the Cdh1GT/GT TS cells and placenta showed aberrant expression of placental differentiation markers. These data highlight an important role of Cdh1 in the G2/M transition during placental differentiation.",
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AU - Naoe, Hideaki

AU - Chiyoda, Tatsuyuki

AU - Ishizawa, Jo

AU - Masuda, Kenta

AU - Saya, Hideyuki

AU - Kuninaka, Shinji

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AB - Differentiation of placental trophoblast stem (TS) cells to trophoblast giant (TG) cells is accompanied by transition from a mitotic cell cycle to an endocycle. Here, we report that Cdh1, a regulator of the anaphase-promoting complex/cyclosome (APC/C), negatively regulates mitotic entry upon the mitotic/endocycle transition. TS cells derived from homozygous Cdh1 gene-trapped (Cdh1GT/GT) murine embryos accumulated mitotic cyclins and precociously entered mitosis after induction of TS cell differentiation, indicating that Cdh1 is required for the switch from mitosis to the endocycle. Furthermore, the Cdh1GT/GT TS cells and placenta showed aberrant expression of placental differentiation markers. These data highlight an important role of Cdh1 in the G2/M transition during placental differentiation.

KW - APC/C

KW - Cdh1

KW - Endocycle

KW - G2/M transition

KW - Trophoblast giant cell

KW - Trophoblast stem cell

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