The basic helix-loop-helix transcription factor, dHAND, is required for vascular development

Hiroyuki Yamagishi, Eric N. Olson, Deepak Srivastava

研究成果: Article査読

119 被引用数 (Scopus)

抄録

Reciprocal interactions between vascular endothelial cells and vascular mesenchymal cells are essential for angiogenesis. Here we show that the basic helix-loop-helix transcription factor, dHAND/Hand2, is expressed in the developing vascular mesenchyme and its derivative, vascular smooth muscle cells (VSMCs). Targeted deletion of the dHAND gene in mice revealed severe defects of embryonic and yolk sac vascular development by embryonic day 9.5. Vascular endothelial cells expressed most markers of differentiation. Vascular mesenchymal cells migrated appropriately but failed to make contact with vascular endothelial cells and did not differentiate into VSMCs. In a screen for genes whose expression was dependent upon dHAND (using subtractive hybridization comparing wild-type and dHAND-null hearts), the VEGF165 receptor, neuropilin-1, was found to be downregulated in dHAND mutants. These results suggest that dHAND is required for vascular development and regulates angiogenesis, possibly through a VEGF signaling pathway.

本文言語English
ページ(範囲)261-270
ページ数10
ジャーナルJournal of Clinical Investigation
105
3
DOI
出版ステータスPublished - 2000 2月
外部発表はい

ASJC Scopus subject areas

  • 医学(全般)

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