The Blimp1-Bcl6 axis is critical to regulate osteoclast differentiation and bone homeostasis

Yoshiteru Miyauchi, Ken Ninomiya, Hiroya Miyamoto, Akemi Sakamoto, Ryotaro Iwasaki, Hiroko Hoshi, Kana Miyamoto, Wu Hao, Shigeyuki Yoshida, Hideo Morioka, Kazuhiro Chiba, Shigeaki Kato, Takeshi Tokuhisa, Mitinori Saitou, Yoshiaki Toyama, Toshio Suda, Takeshi Miyamoto

研究成果: Article査読

124 被引用数 (Scopus)

抄録

Controlling osteoclastogenesis is critical to maintain physiological bone homeostasis and prevent skeletal disorders. Although signaling activating nuclear factor of activated T cells 1 (NFATc1), a transcription factor essential for osteoclastogenesis, has been intensively investigated, factors antagonistic to NFATc1 in osteoclasts have not been characterized. Here, we describe a novel pathway that maintains bone homeostasis via two transcriptional repressors, B cell lymphoma 6 (Bcl6) and B lymphocyte-induced maturation protein-1 (Blimp1). We show that Bcl6 directly targets 'osteoclastic' molecules such as NFATc1, cathepsin K, and dendritic cell-specific transmembrane protein (DC-STAMP), all of which are targets of NFATc1. Bcl6-overexpression inhibited osteoclastogenesis in vitro, whereas Bcl6-deficient mice showed accelerated osteoclast differentiation and severe osteoporosis. We report that Bcl6 is a direct target of Blimp1 and that mice lacking Blimp1 in osteoclasts exhibit osteopetrosis caused by impaired osteoclastogenesis resulting from Bcl6 up-regulation. Indeed, mice doubly mutant in Blimp1 and Bcl6 in osteoclasts exhibited decreased bone mass with increased osteoclastogenesis relative to osteoclast-specific Blimp1-deficient mice. These results reveal a Blimp1-Bcl6-osteoclastic molecule axis, which critically regulates bone homeostasis by controlling osteoclastogenesis and may provide a molecular basis for novel therapeutic strategies.

本文言語English
ページ(範囲)751-762
ページ数12
ジャーナルJournal of Experimental Medicine
207
4
DOI
出版ステータスPublished - 2010 4 12

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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