The coxsackievirus-adenovirus receptor protein as a cell adhesion molecule in the developing mouse brain

Takao Honda, Hiroshi Saitoh, Masayoshi Masuko, Takako Katagiri-Abe, Kei Tominaga, Ikuo Kozakai, Kazuo Kobayashi, Toshiro Kumanishi, Yuichi G. Watanabe, Shoji Odani, Ryozo Kuwano

研究成果: Article

175 引用 (Scopus)

抄録

In an attempt to elucidate the molecular mechanisms underlying neuro- network formation in the developing brain, we analyzed 130 proteolytic cleavage peptides of membrane proteins purified from newborn mouse brains. We describe here the characterization of a membrane protein with an apparent molecular mass of 46 kDa, a member of the immunoglobulin superfamily of which the cDNA sequence was recently reported, encoding the mouse homologue of the human coxsackievirus and adenovirus receptor (mCAR). Western and Northern blot analyses demonstrated the abundant expression of mCAR in the mouse brain, the highest level being observed in the newborn mouse brain, and its expression was detected in embryos as early as at 10.5 days post-coitus (dpc), but decreased rapidly after birth. On in situ hybridization, mCAR mRNA expression was observed throughout the newborn mouse brain. In primary neurons from the hippocampi of mouse embryos the expression of mCAR was observed throughout the cells including those in growth cones on immunohistochemistry. In order to determine whether or not mCAR is involved in cell adhesion, aggregation assays were carried out. C6 cells transfected with mCAR cDNA aggregated homophilically, which was inhibited by specific antibodies against the extracellular domain of mCAR. In addition to its action as a virus receptor, mCAR may function naturally as an adhesion molecule involved in neuro-network formation in the developing nervous system. (C) 2000 Elsevier Science B.V.

元の言語English
ページ(範囲)19-28
ページ数10
ジャーナルMolecular Brain Research
77
発行部数1
DOI
出版物ステータスPublished - 2000 4 14
外部発表Yes

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Enterovirus
Cell Adhesion Molecules
Brain
Proteins
Membrane Proteins
Embryonic Structures
Complementary DNA
Virus Receptors
Human Adenoviruses
Cell Aggregation
Growth Cones
Coitus
Cell Adhesion
Northern Blotting
Nervous System
In Situ Hybridization
adenovirus receptor
Immunoglobulins
Hippocampus
Western Blotting

ASJC Scopus subject areas

  • Molecular Biology
  • Cellular and Molecular Neuroscience

これを引用

The coxsackievirus-adenovirus receptor protein as a cell adhesion molecule in the developing mouse brain. / Honda, Takao; Saitoh, Hiroshi; Masuko, Masayoshi; Katagiri-Abe, Takako; Tominaga, Kei; Kozakai, Ikuo; Kobayashi, Kazuo; Kumanishi, Toshiro; Watanabe, Yuichi G.; Odani, Shoji; Kuwano, Ryozo.

:: Molecular Brain Research, 巻 77, 番号 1, 14.04.2000, p. 19-28.

研究成果: Article

Honda, T, Saitoh, H, Masuko, M, Katagiri-Abe, T, Tominaga, K, Kozakai, I, Kobayashi, K, Kumanishi, T, Watanabe, YG, Odani, S & Kuwano, R 2000, 'The coxsackievirus-adenovirus receptor protein as a cell adhesion molecule in the developing mouse brain', Molecular Brain Research, 巻. 77, 番号 1, pp. 19-28. https://doi.org/10.1016/S0169-328X(00)00036-X
Honda, Takao ; Saitoh, Hiroshi ; Masuko, Masayoshi ; Katagiri-Abe, Takako ; Tominaga, Kei ; Kozakai, Ikuo ; Kobayashi, Kazuo ; Kumanishi, Toshiro ; Watanabe, Yuichi G. ; Odani, Shoji ; Kuwano, Ryozo. / The coxsackievirus-adenovirus receptor protein as a cell adhesion molecule in the developing mouse brain. :: Molecular Brain Research. 2000 ; 巻 77, 番号 1. pp. 19-28.
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