The cutting edge of histochemical technique: In situ PCR

Yoshiki Hiraoka, Motoyuki Ogawa, Yukinao Sakai, Sadakazu Aiso

研究成果: Article査読

3 被引用数 (Scopus)

抄録

The most innovative technique introduced recently into the field of biological study is the polymerase chain reaction (PCR) which can amplify even single-copy DNA sequences to high levels. In situ PCR, which is a method of in situ amplification of DNA of interest in tissue sections, has been subsequently developed and mainly used to investigate tissues infected with viruses. A combination of reverse transcription and PCR has been utilized to detect mRNA of interest in situ. The technique of in situ PCR is promising for a wide variety of applications in basic histology and histopathology.

本文言語English
ページ(範囲)255-258
ページ数4
ジャーナルActa Histochemica et Cytochemica
31
4
DOI
出版ステータスPublished - 1998 1 1

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Biochemistry
  • Physiology
  • Histology
  • Cell Biology

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