The detergent-insoluble microdomains, rafts, can be used as an effective immunogen

Yohko U. Katagiri, Hideki Nakajima, Ban Sato, Yoshitaka Miyagawa, Yasuomi Horiuchi, Hajime Okita, Junichiro Fujimoto, Nobutaka Kiyokawa

研究成果: Article査読

2 被引用数 (Scopus)

抄録

Detergent-insoluble microdomains, or rafts, act as a platform to transduce signals from the extracellular space into the cytoplasm. In the process of developing monoclonal antibodies against raft molecules for the purpose of studying the molecular mechanism of raft-mediated signaling, we observed the uniqueness and certain advantages of immunization with rafts. Simple subcutaneous injection of mice with a phosphate-buffered saline (PBS) suspension of rafts without mixing with Freund's adjuvant made it possible to increase the titer of antiserum reacting with raft components. Interestingly, injection of rafts prepared from certain specific cell lines induced monoglycolipid-specific antibodies. Furthermore, antibodies were produced by raft-immunization of even syngeneic mice. Our findings suggest that this phenomenon does not represent a breakdown of immunological self-tolerance, but typical immune reactions accompanying the class switch from IgM antibodies to IgG antibodies.

本文言語English
ページ(範囲)495-501
ページ数7
ジャーナルGlycoconjugate Journal
25
6
DOI
出版ステータスPublished - 2008 8月
外部発表はい

ASJC Scopus subject areas

  • 生化学
  • 分子生物学
  • 細胞生物学

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