The Direct Boil-LAMP method: A simple and rapid diagnostic method for cutaneous leishmaniasis

Kei Mikita, Takuya Maeda, Sachio Yoshikawa, Takeshi Ono, Yasushi Miyahira, Akihiko Kawana

研究成果: Article

18 引用 (Scopus)

抄録

Needle biopsy is widely used for the diagnosis of cutaneous leishmaniasis (CL) to obtain specimens for histology and culture. However, the use of such invasive procedures causes discomfort, requires technical expertise, and carries risks of bleeding and iatrogenic infection. Therefore, developing substitutive non-invasive diagnostic tools for CL will help reduce the risk of secondary infection and the exposure of both infected individuals and medical professionals. Here we employed loop-mediated isothermal amplification and boiled swab samples (Direct Boil-LAMP method) from CL model mice to develop a simple and rapid diagnostic method for CL. The detection limit of this procedure was 1.0×103parasites/mL. Accordingly, this substitutive diagnostic method should prove useful for mass screening. In addition, we discuss the potential advantages of using it, particularly in endemic regions where medical resources are limited.

元の言語English
ページ(範囲)785-789
ページ数5
ジャーナルParasitology International
63
発行部数6
DOI
出版物ステータスPublished - 2014
外部発表Yes

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Furunculosis
Cutaneous Leishmaniasis
Professional Competence
Mass Screening
Needle Biopsy
Coinfection
Limit of Detection
Histology
Hemorrhage
Infection

ASJC Scopus subject areas

  • Parasitology
  • Infectious Diseases
  • Medicine(all)

これを引用

The Direct Boil-LAMP method : A simple and rapid diagnostic method for cutaneous leishmaniasis. / Mikita, Kei; Maeda, Takuya; Yoshikawa, Sachio; Ono, Takeshi; Miyahira, Yasushi; Kawana, Akihiko.

:: Parasitology International, 巻 63, 番号 6, 2014, p. 785-789.

研究成果: Article

Mikita, Kei ; Maeda, Takuya ; Yoshikawa, Sachio ; Ono, Takeshi ; Miyahira, Yasushi ; Kawana, Akihiko. / The Direct Boil-LAMP method : A simple and rapid diagnostic method for cutaneous leishmaniasis. :: Parasitology International. 2014 ; 巻 63, 番号 6. pp. 785-789.
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AU - Miyahira, Yasushi

AU - Kawana, Akihiko

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