The dual effects of the astrocyte-specific enhancer of the AQP4 M1 promoter

Yoichiro Abe, Wakami Goda, Hiroko Ikeshima-Kataoka, Masato Yasui

研究成果: Article査読

抄録

Aquaporin-4, a predominant water channel in the central nervous system, has two isoforms, M1 and M23, whose transcripts are driven by distinct promoters. Using a reporter assay, we found that a fragment located between exons 0 and 1 of the mouse aquaporin-4 gene, which had been thought to be the promoter for M23, lacked enhancers functioning in astrocytes. When the astrocyte-specific enhancer (ASE) of the M1 promoter is connected to the putative M23 core promoter, it also works in astrocytes. Importantly, the ASE inhibits downstream promoter activity in NIH3T3 cells, indicating that the ASE also functions as a silencer in cells lacking aquaporin-4.

本文言語English
ページ(範囲)3906-3915
ページ数10
ジャーナルFEBS Letters
591
23
DOI
出版ステータスPublished - 2017 12

ASJC Scopus subject areas

  • 生物理学
  • 構造生物学
  • 生化学
  • 分子生物学
  • 遺伝学
  • 細胞生物学

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