The Dugesia ryukyuensis database as a molecular resource for studying switching of the reproductive system

Hideyuki Ishizuka, Takanobu Maezawa, Junpei Kawauchi, Hanae Nodono, Yukako Hirao, Osamu Nishimura, Haruka Nakagawa, Kiyono Sekii, Kenta Tasaka, Hiroshi Tarui, Kiyokazu Agata, Motonori Hoshi, Kazuya Kobayashi, Yasubumi Sakakibara, Midori Matsumoto

研究成果: Article査読

26 被引用数 (Scopus)

抄録

The planarian Dugesia ryukyuensis reproduces both asexually and sexually, and can switch from one mode of reproduction to the other. We recently developed a method for experimentally switching reproduction of the planarian from the asexual to the sexual mode. We constructed a cDNA library from sexualized D. ryukyuensis and sequenced and analyzed 8,988 expressed sequence tags (ESTs). The ESTs were analyzed and grouped into 3,077 non-redundant sequences, leaving 1,929 singletons that formed the basis of unigene sets. Fifty-six percent of the cDNAs analyzed shared similarity (E-value<1E -20) with sequences deposited in NCBI. Highly redundant sequences encoded granulin and actin, which are expressed in the whole body, and other redundant sequences encoded a Vasa-like protein, which is known to be a component of germ-line cells and is expressed in the ovary, and Y-protein, which is expressed in the testis. The sexualized planarian expressed sequence tag database (http://planaria.bio.keio.ac.jp/ planaria/) is an open-access, online resource providing access to sequence, classification, clustering, and annotation data. This database should constitute a powerful tool for analyzing sexualization in planarians.

本文言語English
ページ(範囲)31-37
ページ数7
ジャーナルZoological Science
24
1
DOI
出版ステータスPublished - 2007 1

ASJC Scopus subject areas

  • 動物科学および動物学

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