The E-cadherin gene is silenced by CpG methylation in human hepatocellular carcinomas

Yae Kanai, Saori Ushijima, Ai Min Hui, Atsushi Ochiai, Hitoshi Tsuda, Michiie Sakamoto, Setsuo Hirohashi

研究成果: Article査読

243 被引用数 (Scopus)

抄録

Our study was designed to clarify the significance of silencing the E- cadherin gene, which is located on 16q22.1, due to CpG methylation during hepatocarcinogenesis. The CpG methylation status of primary hepatocellular carcinomas (HCCs) and corresponding liver tissues showing chronic hepatitis or cirrhosis, which are widely considered to be precancerous conditions, were assessed by digesting DNA with methylation-sensitive and non-sensitive restriction enzymes. CpG methylation around the promoter region of the E- cadherin gene was detected in 46% of liver tissues showing chronic hepatitis or cirrhosis and 67% of HCCs examined. Immunohistochemical examination revealed reduced E-cadherin expression in 59% of HCCs examined. CpG methylation around the promoter region correlated significantly with reduced E-cadherin expression in HCCs (p < 0.05). CpG methylation around the promoter region, which increases during the progression from a precancerous condition to HCC, may participate in hepatocarcinogenesis through reduction of E- cadherin expression, resulting in loss of intercellular adhesiveness and destruction of tissue morphology.

本文言語English
ページ(範囲)355-359
ページ数5
ジャーナルInternational Journal of Cancer
71
3
DOI
出版ステータスPublished - 1997 6月 9
外部発表はい

ASJC Scopus subject areas

  • 腫瘍学
  • 癌研究

フィンガープリント

「The E-cadherin gene is silenced by CpG methylation in human hepatocellular carcinomas」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

引用スタイル