The E3 ligase VHL promotes follicular helper T cell differentiation via glycolytic-epigenetic control

Yangyang Zhu, Yanxia Zhao, Le Zou, Danfeng Zhang, Daisuke Aki, Yun Cai Liu

研究成果: Article査読

7 被引用数 (Scopus)

抄録

Follicular helper T (Tfh) cells are essential for germinal center formation and effective humoral immunity, which undergo different stages of development to become fully polarized. However, the detailed mechanisms of their regulation remain unsolved. Here we found that the E3 ubiquitin ligase VHL was required for Tfh cell development and function upon acute virus infection or antigen immunization. VHL acted through the hypoxia-inducible factor 1α (HIF-1α)−dependent glycolysis pathway to positively regulate early Tfh cell initiation. The enhanced glycolytic activity due to VHL deficiency was involved in the epigenetic regulation of ICOS expression, a critical molecule for Tfh development. By using an RNA interference screen, we identified the glycolytic enzyme GAPDH as the key target for the reduced ICOS expression via m6A modification. Our results thus demonstrated that the VHL-HIF-1α axis played an important role during the initiation of Tfh cell development through glycolytic-epigenetic reprogramming.

本文言語English
ページ(範囲)1664-1681
ページ数18
ジャーナルJournal of Experimental Medicine
216
7
DOI
出版ステータスPublished - 2019

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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