抄録
Our research group aims to develop an osteochondral composite using type II collagen gel with hydroxyapatite (HAp) deposited on one side. Soaking gels in Ca2+ and phosphate solution is indispensable to HAp deposition, so relationships between cell behavior and Ca2+ concentration were examined in two- and three-dimensional cultures. The present results indicate that 2-4 mM Ca2+ is suitable for proliferation and survival of osteoblasts, whereas slightly higher concentrations (6-8 mM) favor osteoblast differentiation and matrix mineralization in both 2- and 3-dimensional cultures. Higher concentrations (>10mM) are cytotoxic. Purely from the perspective of calcium deposition, higher concentrations lead to increased accumulation of Ca2+. Culturing cells in phosphate-containing gel in media with Ca2+ also leads to time-dependent formation of HAp in the gel. Considering the viability of embedded cells, culturing scaffolds in media with Ca2+ concentrations around 5 mM is useful for both HAp deposition and osteoblast behavior.
本文言語 | English |
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ページ(範囲) | 4847-4855 |
ページ数 | 9 |
ジャーナル | Biomaterials |
巻 | 26 |
号 | 23 |
DOI | |
出版ステータス | Published - 2005 8月 |
ASJC Scopus subject areas
- バイオエンジニアリング
- セラミックおよび複合材料
- 生物理学
- 生体材料
- 材料力学