The Enteropathogenic E. coli Effector EspB Facilitates Microvillus Effacing and Antiphagocytosis by Inhibiting Myosin Function

Yosuke Iizumi, Hiroshi Sagara, Yasuaki Kabe, Motoki Azuma, Kanako Kume, Michinaga Ogawa, Takeshi Nagai, Peter G. Gillespie, Chihiro Sasakawa, Hiroshi Handa

研究成果: Article

67 引用 (Scopus)

抄録

Enteropathogenic Escherichia coli (EPEC) destroys intestinal microvilli and suppresses phagocytosis by injecting effectors into infected cells through a type III secretion system (TTSS). EspB, a component of the TTSS, is also injected into the cytoplasm of host cells. However, the physiological functions of EspB within the host cell cytoplasm remain unclear. We show that EspB binds to myosins, which are a superfamily of proteins that interact with actin filaments and mediate essential cellular processes, including microvillus formation and phagocytosis. EspB inhibits the interaction of myosins with actin, and an EspB mutant that lacks the myosin-binding region maintained its TTSS function but could not induce microvillus effacing or suppress phagocytosis. Moreover, the myosin-binding region of EspB is essential for Citrobacter rodentium, an EPEC-related murine pathogen, to efficiently infect mice. These results suggest that EspB inhibits myosin functions and thereby facilitates efficient infection by EPEC.

元の言語English
ページ(範囲)383-392
ページ数10
ジャーナルCell Host and Microbe
2
発行部数6
DOI
出版物ステータスPublished - 2007 12 13
外部発表Yes

Fingerprint

Enteropathogenic Escherichia coli
Myosins
Microvilli
Phagocytosis
Citrobacter rodentium
Cytoplasm
Actin Cytoskeleton
Actins
Infection
Type III Secretion Systems
Proteins

ASJC Scopus subject areas

  • Immunology and Microbiology(all)
  • Cancer Research
  • Molecular Biology

これを引用

The Enteropathogenic E. coli Effector EspB Facilitates Microvillus Effacing and Antiphagocytosis by Inhibiting Myosin Function. / Iizumi, Yosuke; Sagara, Hiroshi; Kabe, Yasuaki; Azuma, Motoki; Kume, Kanako; Ogawa, Michinaga; Nagai, Takeshi; Gillespie, Peter G.; Sasakawa, Chihiro; Handa, Hiroshi.

:: Cell Host and Microbe, 巻 2, 番号 6, 13.12.2007, p. 383-392.

研究成果: Article

Iizumi, Y, Sagara, H, Kabe, Y, Azuma, M, Kume, K, Ogawa, M, Nagai, T, Gillespie, PG, Sasakawa, C & Handa, H 2007, 'The Enteropathogenic E. coli Effector EspB Facilitates Microvillus Effacing and Antiphagocytosis by Inhibiting Myosin Function', Cell Host and Microbe, 巻. 2, 番号 6, pp. 383-392. https://doi.org/10.1016/j.chom.2007.09.012
Iizumi, Yosuke ; Sagara, Hiroshi ; Kabe, Yasuaki ; Azuma, Motoki ; Kume, Kanako ; Ogawa, Michinaga ; Nagai, Takeshi ; Gillespie, Peter G. ; Sasakawa, Chihiro ; Handa, Hiroshi. / The Enteropathogenic E. coli Effector EspB Facilitates Microvillus Effacing and Antiphagocytosis by Inhibiting Myosin Function. :: Cell Host and Microbe. 2007 ; 巻 2, 番号 6. pp. 383-392.
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