@article{98bc60b432384869bd8efd70fc44cf40,
title = "The first Hes1 dimer inhibitors from natural products",
abstract = "In the present study, we developed a high-throughput screening system for small molecule-inhibitors of the basic helix-loop-helix (bHLH) transcriptional repressor factor Hes1. Successful dimerization of Hes1 immobilized on a microplate and fluorophore (Cy3)-labelled Hes1 was confirmed. Using this system, several natural products were identified as the first Hes1 dimer inhibitors. Of these, two compounds which were isolated from myxomycetes (true slime molds) inhibited Hes1 from N box-dependent suppression of the gene expression in C3H10T1/2 cells.",
keywords = "Hes1, High-throughput screening, Inhibitor, Neural stem cells, bHLH",
author = "Arai, {Midori A.} and Ayako Masada and Toshiyuki Ohtsuka and Ryoichiro Kageyama and Masami Ishibashi",
note = "Funding Information: This study was supported by a Grant-in-Aid for Young Scientists (A) (to M.A.A.) and in part by Grants-in-Aid for Scientific Research (B) (to M.I.) from Japan Society for the Promotion of Science (JSPS). M.A.A. also gratefully acknowledges Uehara Memorial Foundation for financial support. This work was also supported in part by the Hamaguchi Foundation for the Advancement of Biochemistry to M.A.A. Copyright: Copyright 2011 Elsevier B.V., All rights reserved.",
year = "2009",
month = oct,
day = "1",
doi = "10.1016/j.bmcl.2009.07.146",
language = "English",
volume = "19",
pages = "5778--5781",
journal = "Bioorganic and Medicinal Chemistry Letters",
issn = "0960-894X",
publisher = "Elsevier Limited",
number = "19",
}