The Genomic Damage Estimated by Arbitrarily Primed PCR DNA Fingerprinting Is Useful for the Prognosis of Gastric Cancer

Koichi Suzuki, Sumiko Ohnami, Chikako Tanabe, Hiroki Sasaki, Jun Yasuda, Hitoshi Katai, Kimio Yoshimura, Masaaki Terada, Manuel Perucho, Teruhiko Yoshida

研究成果: Article

24 引用 (Scopus)

抄録

Background & Aims: Genomic instability and the accompanying alteration of cancer genes play a major role in tumorigenesis. We evaluated the prognostic significance in gastric cancer of the degree of accumulation of relative genomic damage, assessed by arbitrarily primed polymerase chain reaction DNA fingerprinting. Methods: Genomic damage was assessed by comparative analysis of paired normal and tumor tissue DNA fingerprints. The total number of alterations, scored as decreases and increases of band intensity with 2 arbitrary primers, were used as an estimation of the genomic damage fraction in 74 primary gastric cancers. Increases in DNA copy number were also analyzed by array comparative genomic hybridization in a subset of 30 cases. Results: The number of altered bands varied among the tumors from none or a few to more than one third of the approximately 40 fingerprint bands. The relative values of genomic damage were consistent with the quantitative chromosomal alterations observed by array comparative genomic hybridization. When the tumors were stratified into 2 groups-above or below the cutoff of 0.22 for average genomic damage fraction-genomic damage fraction was a valuable prognostic indicator regardless of microsatellite instability status. Multivariate Cox analysis showed that the genomic damage fraction was a prognostic indicator, as well as a stage indicator (P = 0.0189). Survival was significantly diminished in tumors with a genomic damage fraction >0.22 (P = 0.0009). Moreover, in the 46 curative cases, genomic damage fraction was the only independent factor for predicting survival (P = 0.0061). Conclusions: Our results indicate that the degree of genomic damage estimated by arbitrarily primed polymerase chain reaction fingerprinting is a useful prognostic indicator for gastric cancer.

元の言語English
ページ(範囲)1330-1340
ページ数11
ジャーナルGastroenterology
125
発行部数5
DOI
出版物ステータスPublished - 2003 11
外部発表Yes

Fingerprint

DNA Fingerprinting
Stomach Neoplasms
Polymerase Chain Reaction
Comparative Genomic Hybridization
Neoplasms
Microsatellite Instability
Genomic Instability
Neoplasm Genes
Dermatoglyphics
Carcinogenesis
Multivariate Analysis
DNA

ASJC Scopus subject areas

  • Gastroenterology

これを引用

The Genomic Damage Estimated by Arbitrarily Primed PCR DNA Fingerprinting Is Useful for the Prognosis of Gastric Cancer. / Suzuki, Koichi; Ohnami, Sumiko; Tanabe, Chikako; Sasaki, Hiroki; Yasuda, Jun; Katai, Hitoshi; Yoshimura, Kimio; Terada, Masaaki; Perucho, Manuel; Yoshida, Teruhiko.

:: Gastroenterology, 巻 125, 番号 5, 11.2003, p. 1330-1340.

研究成果: Article

Suzuki, K, Ohnami, S, Tanabe, C, Sasaki, H, Yasuda, J, Katai, H, Yoshimura, K, Terada, M, Perucho, M & Yoshida, T 2003, 'The Genomic Damage Estimated by Arbitrarily Primed PCR DNA Fingerprinting Is Useful for the Prognosis of Gastric Cancer', Gastroenterology, 巻. 125, 番号 5, pp. 1330-1340. https://doi.org/10.1016/j.gastro.2003.07.006
Suzuki, Koichi ; Ohnami, Sumiko ; Tanabe, Chikako ; Sasaki, Hiroki ; Yasuda, Jun ; Katai, Hitoshi ; Yoshimura, Kimio ; Terada, Masaaki ; Perucho, Manuel ; Yoshida, Teruhiko. / The Genomic Damage Estimated by Arbitrarily Primed PCR DNA Fingerprinting Is Useful for the Prognosis of Gastric Cancer. :: Gastroenterology. 2003 ; 巻 125, 番号 5. pp. 1330-1340.
@article{78c8f415a7114a46a40678e4a1200a55,
title = "The Genomic Damage Estimated by Arbitrarily Primed PCR DNA Fingerprinting Is Useful for the Prognosis of Gastric Cancer",
abstract = "Background & Aims: Genomic instability and the accompanying alteration of cancer genes play a major role in tumorigenesis. We evaluated the prognostic significance in gastric cancer of the degree of accumulation of relative genomic damage, assessed by arbitrarily primed polymerase chain reaction DNA fingerprinting. Methods: Genomic damage was assessed by comparative analysis of paired normal and tumor tissue DNA fingerprints. The total number of alterations, scored as decreases and increases of band intensity with 2 arbitrary primers, were used as an estimation of the genomic damage fraction in 74 primary gastric cancers. Increases in DNA copy number were also analyzed by array comparative genomic hybridization in a subset of 30 cases. Results: The number of altered bands varied among the tumors from none or a few to more than one third of the approximately 40 fingerprint bands. The relative values of genomic damage were consistent with the quantitative chromosomal alterations observed by array comparative genomic hybridization. When the tumors were stratified into 2 groups-above or below the cutoff of 0.22 for average genomic damage fraction-genomic damage fraction was a valuable prognostic indicator regardless of microsatellite instability status. Multivariate Cox analysis showed that the genomic damage fraction was a prognostic indicator, as well as a stage indicator (P = 0.0189). Survival was significantly diminished in tumors with a genomic damage fraction >0.22 (P = 0.0009). Moreover, in the 46 curative cases, genomic damage fraction was the only independent factor for predicting survival (P = 0.0061). Conclusions: Our results indicate that the degree of genomic damage estimated by arbitrarily primed polymerase chain reaction fingerprinting is a useful prognostic indicator for gastric cancer.",
author = "Koichi Suzuki and Sumiko Ohnami and Chikako Tanabe and Hiroki Sasaki and Jun Yasuda and Hitoshi Katai and Kimio Yoshimura and Masaaki Terada and Manuel Perucho and Teruhiko Yoshida",
year = "2003",
month = "11",
doi = "10.1016/j.gastro.2003.07.006",
language = "English",
volume = "125",
pages = "1330--1340",
journal = "Gastroenterology",
issn = "0016-5085",
publisher = "W.B. Saunders Ltd",
number = "5",

}

TY - JOUR

T1 - The Genomic Damage Estimated by Arbitrarily Primed PCR DNA Fingerprinting Is Useful for the Prognosis of Gastric Cancer

AU - Suzuki, Koichi

AU - Ohnami, Sumiko

AU - Tanabe, Chikako

AU - Sasaki, Hiroki

AU - Yasuda, Jun

AU - Katai, Hitoshi

AU - Yoshimura, Kimio

AU - Terada, Masaaki

AU - Perucho, Manuel

AU - Yoshida, Teruhiko

PY - 2003/11

Y1 - 2003/11

N2 - Background & Aims: Genomic instability and the accompanying alteration of cancer genes play a major role in tumorigenesis. We evaluated the prognostic significance in gastric cancer of the degree of accumulation of relative genomic damage, assessed by arbitrarily primed polymerase chain reaction DNA fingerprinting. Methods: Genomic damage was assessed by comparative analysis of paired normal and tumor tissue DNA fingerprints. The total number of alterations, scored as decreases and increases of band intensity with 2 arbitrary primers, were used as an estimation of the genomic damage fraction in 74 primary gastric cancers. Increases in DNA copy number were also analyzed by array comparative genomic hybridization in a subset of 30 cases. Results: The number of altered bands varied among the tumors from none or a few to more than one third of the approximately 40 fingerprint bands. The relative values of genomic damage were consistent with the quantitative chromosomal alterations observed by array comparative genomic hybridization. When the tumors were stratified into 2 groups-above or below the cutoff of 0.22 for average genomic damage fraction-genomic damage fraction was a valuable prognostic indicator regardless of microsatellite instability status. Multivariate Cox analysis showed that the genomic damage fraction was a prognostic indicator, as well as a stage indicator (P = 0.0189). Survival was significantly diminished in tumors with a genomic damage fraction >0.22 (P = 0.0009). Moreover, in the 46 curative cases, genomic damage fraction was the only independent factor for predicting survival (P = 0.0061). Conclusions: Our results indicate that the degree of genomic damage estimated by arbitrarily primed polymerase chain reaction fingerprinting is a useful prognostic indicator for gastric cancer.

AB - Background & Aims: Genomic instability and the accompanying alteration of cancer genes play a major role in tumorigenesis. We evaluated the prognostic significance in gastric cancer of the degree of accumulation of relative genomic damage, assessed by arbitrarily primed polymerase chain reaction DNA fingerprinting. Methods: Genomic damage was assessed by comparative analysis of paired normal and tumor tissue DNA fingerprints. The total number of alterations, scored as decreases and increases of band intensity with 2 arbitrary primers, were used as an estimation of the genomic damage fraction in 74 primary gastric cancers. Increases in DNA copy number were also analyzed by array comparative genomic hybridization in a subset of 30 cases. Results: The number of altered bands varied among the tumors from none or a few to more than one third of the approximately 40 fingerprint bands. The relative values of genomic damage were consistent with the quantitative chromosomal alterations observed by array comparative genomic hybridization. When the tumors were stratified into 2 groups-above or below the cutoff of 0.22 for average genomic damage fraction-genomic damage fraction was a valuable prognostic indicator regardless of microsatellite instability status. Multivariate Cox analysis showed that the genomic damage fraction was a prognostic indicator, as well as a stage indicator (P = 0.0189). Survival was significantly diminished in tumors with a genomic damage fraction >0.22 (P = 0.0009). Moreover, in the 46 curative cases, genomic damage fraction was the only independent factor for predicting survival (P = 0.0061). Conclusions: Our results indicate that the degree of genomic damage estimated by arbitrarily primed polymerase chain reaction fingerprinting is a useful prognostic indicator for gastric cancer.

UR - http://www.scopus.com/inward/record.url?scp=0242658711&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0242658711&partnerID=8YFLogxK

U2 - 10.1016/j.gastro.2003.07.006

DO - 10.1016/j.gastro.2003.07.006

M3 - Article

C2 - 14598249

AN - SCOPUS:0242658711

VL - 125

SP - 1330

EP - 1340

JO - Gastroenterology

JF - Gastroenterology

SN - 0016-5085

IS - 5

ER -