The genomic structure and promoter region of the human Parkin gene

S. Asakawa, K. I. Tsunematsu, A. Takayanagi, T. Sasaki, A. Shimizu, A. Shintani, K. Kawasaki, A. J. Mungall, S. Beck, S. Minoshima, N. Shimizu

研究成果: Article査読

39 被引用数 (Scopus)

抄録

Parkin has been identified as a causative gene of the autosomal recessive juvenile parkinsonism (AR-JP). In this study, we determined the genomic structure of the Parkin gene and identified a core promoter region based on the DNA sequence of 1.4 Mb. The 5′-flanking region contained no apparent TATA or CAAT box elements but several putative cis-elements for various transcription factors. The GC- and CpG-rich regions were observed not only in the 5′-flanking sequence but also in the 5′-part of the first intron of Parkin. We identified an exact starting point of Parkin transcription. A core promoter region was determined by transfecting a series of deletion constructs with a dual luciferase reporter system into human neuroblastoma cells. Furthermore, we located a neighboring novel gene in a head-to-head direction with Parkin with only a 198-bp interval.

本文言語English
ページ(範囲)863-868
ページ数6
ジャーナルBiochemical and Biophysical Research Communications
286
5
DOI
出版ステータスPublished - 2001

ASJC Scopus subject areas

  • 生物理学
  • 生化学
  • 分子生物学
  • 細胞生物学

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