The hiiragi gene encodes a poly(A) polymerase, which controls the formation of the wing margin in Drosophila melanogaster

Takehide Murata, Hideyuki Nagaso, Shin ichi Kashiwabara, Tadashi Baba, Hideyuki Okano, Kazunari K. Yokoyama

研究成果: Article査読

17 被引用数 (Scopus)

抄録

The hiiragi (hrg) gene plays a key role in the development of the wing margin in Drosophila melanogaster. A mutation in the hrg gene resulted in a decrease in the level of the hrg transcript and was associated with a notched wing phenotype. We report here that the hrg gene encodes a poly(A) polymerase(PAP). The bovine cDNA for PAP type II reversed the phenotype due to mutation of the hrg gene, suggesting that hrg might encode a functional homolog of PAP. A mutation that reduced the enzymatic activity of Hrg failed to reverse the phenotype of hrg mutants, suggesting that the enzymatic activity of Hrg was required to rescue the wing phenotype. The levels of expression of wingless and cut at the presumptive wing margins were reduced in the late third-instar larvae of hrg mutants. These results suggest that the product of hrg is required for the normal expression of a series of genes in this region. Our results provide the first evidence that a PAP in Drosophila plays a key role in the early development of the wing margin, acting to regulate the specific expression of a series of genes via, perhaps, control of the processing of the 3′ ends of transcripts.

本文言語English
ページ(範囲)137-147
ページ数11
ジャーナルDevelopmental Biology
233
1
DOI
出版ステータスPublished - 2001 5 1
外部発表はい

ASJC Scopus subject areas

  • 分子生物学
  • 発生生物学
  • 細胞生物学

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