@article{9364968696ce4c03ae9703873065ca5f,
title = "The human lipodystrophy protein seipin is an ER membrane adaptor for the adipogenic PA phosphatase lipin 1",
abstract = "Disruption of the gene BSCL2 causes a severe, generalised lipodystrophy, demonstrating the critical role of its protein product, seipin, in human adipose tissue development. Seipin is essential for adipocyte differentiation, whilst the study of seipin in non-adipose cells has suggested a role in lipid droplet formation. However, its precise molecular function remains poorly understood. Here we demonstrate that seipin can inducibly bind lipin 1, a phosphatidic acid (PA) phosphatase important for lipid synthesis and adipogenesis. Knockdown of seipin during early adipogenesis decreases the association of lipin 1 with membranes and increases the accumulation of its substrate PA. Conversely, PA levels are reduced in differentiating cells by overexpression of wild-type seipin but not by expression of a mutated seipin that is unable to bind lipin 1. Together our data identify lipin as the first example of a seipin-interacting protein and reveals a novel molecular function for seipin in developing adipocytes.",
keywords = "Adipogenesis, Endoplasmic reticulum, Lipin, Lipodystrophy, Seipin",
author = "Sim, {M. F.Michelle} and Dennis, {Rowena J.} and Aubry, {Evelyne M.} and Nardev Ramanathan and Hiroshi Sembongi and Vladimir Saudek and Daisuke Ito and Stephen O'Rahilly and Symeon Siniossoglou and Rochford, {Justin J.}",
note = "Funding Information: This work was supported by the Medical Research Council (MRC) [New Investigator Research Grant number GO800203 (to J.J.R.), Senior Fellowship number G0701446 (to S.S), Program Grant number G09000554 (to S.O.R)], the Swiss National Science Foundation [Grant PBBEP3-123654/PA00P3-129106 (to E.M.A)], the Wellcome Trust [Grant number 078986/Z/06/Z (to S.O.R.)], the Agency for Science, Technology and Research , Singapore(A⁎STAR) (N.R.), the MRC Centre for Obesity and Related Metabolic Disorders (MRC-CORD) [Grant number GO600717 ] and the National Institute for Health Research Comprehensive Biomedical Research Centre [Grant number CG50826 ]. ",
year = "2013",
month = feb,
doi = "10.1016/j.molmet.2012.11.002",
language = "English",
volume = "2",
pages = "38--46",
journal = "Molecular Metabolism",
issn = "2212-8778",
publisher = "Elsevier GmbH",
number = "1",
}