The levels of leukemia inhibitory factor in synovial tissues of patients with rheumatoid arthritis: Inflammation and other proinflammatory cytokines

Hiroyuki Enomoto, Seiji Saito, Hiroshi Yabe, Yoshiaki Toyama, Taisuke Tamatu

研究成果: Article査読

5 被引用数 (Scopus)

抄録

To clarify the effect of leukemia inhibitory factor (LIF) on the destruction of rheumatoid arthritis (RA) joints, we investigated the production of LIF and the expression of LIF mRNA in synovial tissues from patients with RA and osteoarthritis (OA). Synovial fluids from RA were used to measure the LIF concentrations using enzyme-linked immunosorbent assay (ELISA). Immunohistochemistory and RT-PCR were used to examine the expression of LIF by synovial cells. LIF mRNA was detected in all cases in RA synovial cells. Although LIF protein was detected only in 20 cases (19%) in RA synovial fluids, LIF concentration in the synovial fluids significantly correlated with the peripheral leukocyte count (P < 0.001) and Greactive protein (CRP) (P < 0.01). Moreover, levels of IL-1β, IL-6, and IL-8, but not TNF-α, were significantly correlated with LIF in the RA synovial fluids. LIF production was promoted by IL-1β and TNF-α stimulation; in contrast, IL-1 ra and IL-4 were found to markedly decrease LIF production by cultured synovial cells. LIF appeared to be a cytokine produced by RA synovium leading to a proinflammatory secretion profile. Moreover, IL-4 and IL-1 ra may represent attenuated activity for reducing the effect of the destruction of joints by LIF.

本文言語English
ページ(範囲)121-128
ページ数8
ジャーナルModern rheumatology
13
2
DOI
出版ステータスPublished - 2003

ASJC Scopus subject areas

  • リウマチ学

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