The mechanism of PDT-induced electrical blockade: The measurement of intracellular Ca2+ concentration changes in cardiac myocytes

A. Ito, S. Hosokawa, S. Hakomori, S. Miyoshi, K. Soejima, T. Arai

研究成果: Conference contribution

4 被引用数 (Scopus)

抄録

We propose the application of early state photodynamic therapy (PDT) to treatment of atrial fibrillation, which is a kind of arrhythmia characterized by irregular rapid beating of heart. We had demonstrated that our PDT can block the propagation of electrical excitation in cardiac myocytes. However, the mechanism of the PDT-induced electrical blockade was not clear. In order to clarify this mechanism, changes in intracellular Ca2+ concentration during the PDT with Talaporfin sodium (water soluble photosensitizer) were measured by fluorescence Ca2+ indicator, fluo-4 AM. The PDT led to the rapid increase of intracellular Ca2+ concentration and the changes in cell shapes. These results indicated that extracellular Ca 2+ flowed into the cells mediated by cell membrane. Moreover, we found bubble generation in the cells after the PDT. In conclusion, the PDT-induced electrical blockade in myocytes can be caused by cell death following the bubble generation, which is accompanied by the increase in intracellular Ca2+ concentration due to the cell membrane malfunction with the PDT.

本文言語English
ホスト出版物のタイトルOptical Interactions with Tissue and Cells XIX
DOI
出版ステータスPublished - 2008 4月 21
イベントOptical Interactions with Tissue and Cells XIX - San Jose, CA, United States
継続期間: 2008 1月 212008 1月 23

出版物シリーズ

名前Progress in Biomedical Optics and Imaging - Proceedings of SPIE
6854
ISSN(印刷版)1605-7422

Other

OtherOptical Interactions with Tissue and Cells XIX
国/地域United States
CitySan Jose, CA
Period08/1/2108/1/23

ASJC Scopus subject areas

  • 電子材料、光学材料、および磁性材料
  • 生体材料
  • 原子分子物理学および光学
  • 放射線学、核医学およびイメージング

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