The murine stk gene product, a transmembrane protein tyrosine kinase, is a receptor for macrophage-stimulating protein

Ming Hai Wang, Atsushi Iwama, Alison Skeel, Toshio Suda, Edward J. Leonard

研究成果: Article

71 被引用数 (Scopus)

抄録

Macrophage-stimulating protein (MSP) was originally identified as an inducer of murine resident peritoneal macrophage responsiveness to chemoattractants. We recently showed that the product of RON, a protein tyrosine kinase cloned from a human keratinocyte library, is the receptor for MSP. Similarity of murine stk to RON led us to determine if the stk gene product is the murine receptor for MSP. Radiolabeled MSP could bind to NIH 3T3 cells transfected with murine stk cDNA (3T3/stk). Binding was saturable and was inhibited by unlabeled MSP but not by structurally related proteins, including hepatocyte growth factor and plasminogen. Specific binding to STK was demonstrated by cross-linking of 125I-labeled MSP to membrane proteins of 3T3/stk cells, which resulted in a protein complex with a molecular mass of 220 kDa. This radiolabeled complex comprised 125I-MSP and STK, since it could be immunoprecipitated by antibodies to the STK β chain. Binding of MSP to stk cDNA-transfected cells induced tyrosine phosphorylation of the 150- kDa STK β chain within I min and caused increased motile activity. These results establish the murine stk gene product as a specific transmembrane protein tyrosine kinase receptor for MSP. Inasmuch as the stk cDNA was cloned from a hematopoietic stem cell, our data suggest that in addition to macrophages and keratinocytes, a cell in the hematopoietic lineage may also be a target for MSP.

本文言語English
ページ(範囲)3933-3937
ページ数5
ジャーナルProceedings of the National Academy of Sciences of the United States of America
92
9
DOI
出版ステータスPublished - 1995 4 25

ASJC Scopus subject areas

  • General

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