The new application of photosensitization reaction to atrial fibrillation treatment: Mechanism and demonstration of non-thermal electrical conduction block with porcine heart

Arisa Ito, Hiroki Matsuo, Tsukasa Suenari, Takuro Kajihara, Takehiro Kimura, Shunichiro Miyoshi, Satoshi Ogawa, Tsunenori Arai

研究成果: Conference contribution

2 被引用数 (Scopus)

抄録

We have proposed non-thermal electrical conduction block for atrial fibrillation treatment by the photosensitization reaction, in which the interval time between the photosensitizer injection and irradiation is less than tenth of that in conventional way. To study the mechanism of photosensitization reaction-induced electrical conduction block, intracellular Ca2+ concentration change in rat myocardial cells was measured by fluorescent Ca 2+ indicator Fluo-4 AM with confocal laser microscopy. Measured rapid increase in the fluorescence intensity and a change in cell morphology indicated that cell membrane damage; that is Ca2+ influx and eventually cell death caused by the photosensitization reaction. To demonstrate myocardial electrical conduction block induced by the photosensitization reaction, surgically exposed porcine heart under deep anesthesia was used. The myocardial tissue was paced with a stimulation electrode. The propagated electrical signals were measured by bipolar electrodes at two different positions. Thirty minutes after the injection of 5-10 mg/kg Porfimer sodium or Talaporfin sodium, the red laser light was irradiated to the tissue point by point crossing the measuring positions by the total energy density of less than 200 J/cm2. The electrical signal conduction between the measuring electrodes in the myocardial tissue was delayed by each irradiation procedure. The electrical conduction delay corresponded to the block line length was obtained. These results demonstrated the possibility of non-thermal electrical conduction block for atrial fibrillation treatment by the photosensitization reaction.

本文言語English
ホスト出版物のタイトル12th World Congress of the International Photodynamic Association - Photodynamic Therapy
ホスト出版物のサブタイトルBack to the Future
DOI
出版ステータスPublished - 2009 12 1
イベント12th World Congress of the International Photodynamic Association - Photodynamic Therapy: Back to the Future - Seattle, WA, United States
継続期間: 2009 6 112009 6 15

出版物シリーズ

名前Progress in Biomedical Optics and Imaging - Proceedings of SPIE
7380
ISSN(印刷版)1605-7422

Other

Other12th World Congress of the International Photodynamic Association - Photodynamic Therapy: Back to the Future
国/地域United States
CitySeattle, WA
Period09/6/1109/6/15

ASJC Scopus subject areas

  • 電子材料、光学材料、および磁性材料
  • 生体材料
  • 原子分子物理学および光学
  • 放射線学、核医学およびイメージング

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