TY - JOUR
T1 - The RNA silencing technology applied by lentiviral vectors in oncology.
AU - Sumimoto, Hidetoshi
AU - Kawakami, Yutaka
PY - 2010
Y1 - 2010
N2 - Since the discovery of RNA interference (RNAi) in Caenorhabditis elegans in 1998, this mechanism has been found to be conserved in a wide variety of species, including insects, plants, and mammals. In mammals, small (or short) interfering RNA (siRNA) or short hairpin RNA (shRNA) can be expressed by using several expression vectors including lentiviral vectors. The lentiviral vector has several useful characteristics for RNAi experiments including broad host tropism and stable gene transduction to both dividing and nondividing cells, which permits stable depletion of target genes. This technology can be useful for several applications, including basic cancer research.
AB - Since the discovery of RNA interference (RNAi) in Caenorhabditis elegans in 1998, this mechanism has been found to be conserved in a wide variety of species, including insects, plants, and mammals. In mammals, small (or short) interfering RNA (siRNA) or short hairpin RNA (shRNA) can be expressed by using several expression vectors including lentiviral vectors. The lentiviral vector has several useful characteristics for RNAi experiments including broad host tropism and stable gene transduction to both dividing and nondividing cells, which permits stable depletion of target genes. This technology can be useful for several applications, including basic cancer research.
UR - http://www.scopus.com/inward/record.url?scp=77953920564&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=77953920564&partnerID=8YFLogxK
U2 - 10.1007/978-1-60761-533-0_13
DO - 10.1007/978-1-60761-533-0_13
M3 - Article
C2 - 20225045
AN - SCOPUS:77953920564
SN - 1064-3745
VL - 614
SP - 187
EP - 199
JO - Methods in Molecular Biology
JF - Methods in Molecular Biology
ER -
