The role of a real-time PCR technology for rapid detection and identification of bacterial and fungal pathogens in whole-blood samples

Hideaki Obara, Naoki Aikawa, Naoki Hasegawa, Shingo Hori, Yasuo Ikeda, Yoshio Kobayashi, Mitsuru Murata, Shinichiro Okamoto, Junzo Takeda, Minoru Tanabe, Yasuhiko Sakakura, Hiroyuki Ginba, Masaki Kitajima, Yuukou Kitagawa

研究成果: Article

16 引用 (Scopus)

抄録

The rapid diagnosis of pathogens and prompt initiation of appropriate antibiotic therapy are critical factors to reduce the morbidity and mortality associated with sepsis. In this study, we evaluated a multiplex polymerase chain reaction (PCR-M) test that detects bacteria and fungi in whole-blood specimens, comparing its features to those of a blood culture (BC). Following evaluation of the performance for sensitivity and specificity of PCR-M, 78 blood samples from 54 patients with suspected bacterial infections were evaluated. Whole-blood samples for PCR-M were collected at the same time as BC, and PCR-M results were compared with BC results. As a result, minimum sensitivity of the kit was 1-100 cfu/ml. The PCR-M test correctly identified specificity for 13 out of 14 strains blinded to the assay analyst. Of 78 blood samples examined, 56 (72%) were negative by both methods, and 22 (28%) were positive by at least one of the two methods. PCR-M detected organisms in 21 cases (27%) compared with 12 cases (15%) in BC. The correlation of positives between PCR-M and BC was 92% (11/12), and both methods identified the same organisms in these 11 cases. With higher positive rate compared with BC, PCR-M could detect and identify potentially significant microorganisms within a few hours by using a small volume of a single whole-blood sample. Early detection of microorganisms has the potential to facilitate early determination of appropriate treatment and antimicrobial selection.

元の言語English
ページ(範囲)327-333
ページ数7
ジャーナルJournal of Infection and Chemotherapy
17
発行部数3
DOI
出版物ステータスPublished - 2011 6

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Real-Time Polymerase Chain Reaction
Technology
Polymerase Chain Reaction
Multiplex Polymerase Chain Reaction
Bacterial Infections
Blood Culture
Sepsis
Fungi
Anti-Bacterial Agents
Morbidity
Bacteria
Sensitivity and Specificity
Mortality
Therapeutics

ASJC Scopus subject areas

  • Microbiology (medical)
  • Pharmacology (medical)
  • Infectious Diseases

これを引用

The role of a real-time PCR technology for rapid detection and identification of bacterial and fungal pathogens in whole-blood samples. / Obara, Hideaki; Aikawa, Naoki; Hasegawa, Naoki; Hori, Shingo; Ikeda, Yasuo; Kobayashi, Yoshio; Murata, Mitsuru; Okamoto, Shinichiro; Takeda, Junzo; Tanabe, Minoru; Sakakura, Yasuhiko; Ginba, Hiroyuki; Kitajima, Masaki; Kitagawa, Yuukou.

:: Journal of Infection and Chemotherapy, 巻 17, 番号 3, 06.2011, p. 327-333.

研究成果: Article

Obara, Hideaki ; Aikawa, Naoki ; Hasegawa, Naoki ; Hori, Shingo ; Ikeda, Yasuo ; Kobayashi, Yoshio ; Murata, Mitsuru ; Okamoto, Shinichiro ; Takeda, Junzo ; Tanabe, Minoru ; Sakakura, Yasuhiko ; Ginba, Hiroyuki ; Kitajima, Masaki ; Kitagawa, Yuukou. / The role of a real-time PCR technology for rapid detection and identification of bacterial and fungal pathogens in whole-blood samples. :: Journal of Infection and Chemotherapy. 2011 ; 巻 17, 番号 3. pp. 327-333.
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AU - Hori, Shingo

AU - Ikeda, Yasuo

AU - Kobayashi, Yoshio

AU - Murata, Mitsuru

AU - Okamoto, Shinichiro

AU - Takeda, Junzo

AU - Tanabe, Minoru

AU - Sakakura, Yasuhiko

AU - Ginba, Hiroyuki

AU - Kitajima, Masaki

AU - Kitagawa, Yuukou

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AB - The rapid diagnosis of pathogens and prompt initiation of appropriate antibiotic therapy are critical factors to reduce the morbidity and mortality associated with sepsis. In this study, we evaluated a multiplex polymerase chain reaction (PCR-M) test that detects bacteria and fungi in whole-blood specimens, comparing its features to those of a blood culture (BC). Following evaluation of the performance for sensitivity and specificity of PCR-M, 78 blood samples from 54 patients with suspected bacterial infections were evaluated. Whole-blood samples for PCR-M were collected at the same time as BC, and PCR-M results were compared with BC results. As a result, minimum sensitivity of the kit was 1-100 cfu/ml. The PCR-M test correctly identified specificity for 13 out of 14 strains blinded to the assay analyst. Of 78 blood samples examined, 56 (72%) were negative by both methods, and 22 (28%) were positive by at least one of the two methods. PCR-M detected organisms in 21 cases (27%) compared with 12 cases (15%) in BC. The correlation of positives between PCR-M and BC was 92% (11/12), and both methods identified the same organisms in these 11 cases. With higher positive rate compared with BC, PCR-M could detect and identify potentially significant microorganisms within a few hours by using a small volume of a single whole-blood sample. Early detection of microorganisms has the potential to facilitate early determination of appropriate treatment and antimicrobial selection.

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