TY - JOUR
T1 - The role of p53 mutation in the carcinomas arising from endometriosis
AU - Akahane, Tomoko
AU - Sekizawa, Akihiko
AU - Purwosunu, Yuditiya
AU - Nagatsuka, Masaaki
AU - Okai, Takashi
PY - 2007/7/1
Y1 - 2007/7/1
N2 - To probe the mechanism of the development of ovarian cancer from endometriosis, which is a multistep process that involves the activation of oncogenes and inactivation of tumor suppressor genes, we evaluated p53 mutations in solitary endometriosis and endometriosis coexisting with ovarian clear cell carcinoma (OCCA) and ovarian endometrioid carcinoma (OEC). We examined 7 cases of solitary ovarian endometriosis, 13 cases of OCCA, and 9 cases of OEC. Cancer tissue specimens that also contained endometriosis without atypia were chosen. Using a laser microdissection system, epithelial cells from the areas of endometriosis were isolated, and DNA was extracted. We amplified exons 5, 6, 7, and 8 of the p53 gene, and direct sequencing was performed. p53 mutation was observed in 4 (30.8%) of 13 specimens of endometriosis coexisting with OCCA, whereas no mutations were detected in solitary endometriosis or endometriosis coexisting with OEC. We thought that some genetic alterations, which induce p53 mutations in endometriosis, may affect malignant transformation of endometriosis into OCCA.
AB - To probe the mechanism of the development of ovarian cancer from endometriosis, which is a multistep process that involves the activation of oncogenes and inactivation of tumor suppressor genes, we evaluated p53 mutations in solitary endometriosis and endometriosis coexisting with ovarian clear cell carcinoma (OCCA) and ovarian endometrioid carcinoma (OEC). We examined 7 cases of solitary ovarian endometriosis, 13 cases of OCCA, and 9 cases of OEC. Cancer tissue specimens that also contained endometriosis without atypia were chosen. Using a laser microdissection system, epithelial cells from the areas of endometriosis were isolated, and DNA was extracted. We amplified exons 5, 6, 7, and 8 of the p53 gene, and direct sequencing was performed. p53 mutation was observed in 4 (30.8%) of 13 specimens of endometriosis coexisting with OCCA, whereas no mutations were detected in solitary endometriosis or endometriosis coexisting with OEC. We thought that some genetic alterations, which induce p53 mutations in endometriosis, may affect malignant transformation of endometriosis into OCCA.
KW - Carcinogenesis
KW - Endometriosis
KW - Ovarian clear cell carcinoma
KW - Ovarian endometrial carcinoma
KW - p53
UR - http://www.scopus.com/inward/record.url?scp=34250818145&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=34250818145&partnerID=8YFLogxK
U2 - 10.1097/pgp.0b013e31802b41a8
DO - 10.1097/pgp.0b013e31802b41a8
M3 - Article
C2 - 17581423
AN - SCOPUS:34250818145
VL - 26
SP - 345
EP - 351
JO - International Journal of Gynecological Pathology
JF - International Journal of Gynecological Pathology
SN - 0277-1691
IS - 3
ER -