The stratum corneum comprises three layers with distinct metal-ion barrier properties

Akiharu Kubo, Itsuko Ishizaki, Akiko Kubo, Hiroshi Kawasaki, Keisuke Nagao, Yoshiharu Ohashi, Masayuki Amagai

研究成果: Article査読

34 被引用数 (Scopus)

抄録

The stratum corneum (SC), the outermost barrier of mammalian bodies, consists of layers of cornified keratinocytes with intercellular spaces sealed with lipids. The insolubility of the SC has hampered in-depth analysis, and the SC has been considered a homogeneous barrier. Here, we applied time-of-flight secondary ion mass spectrometry to demonstrate that the SC consists of three layers with distinct properties. Arginine, a major component of filaggrin-derived natural moisturizing factors, was concentrated in the middle layer, suggesting that this layer functions in skin hydration. Topical application of metal ions revealed that the outer layer allowed their passive influx and efflux, while the middle and lower layers exhibited distinct barrier properties, depending on the metal tested. Notably, filaggrin deficiency abrogated the lower layer barrier, allowing specific metal ions to permeate viable layers. These findings elucidate the multi-layered barrier function of the SC and its defects in filaggrin-deficient atopic disease patients.

本文言語English
論文番号1731
ジャーナルScientific reports
3
DOI
出版ステータスPublished - 2013

ASJC Scopus subject areas

  • General

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