抄録
A thermoresponsive copolymer incorporating a quaternary amine group, poly(N-isopropylacrylamide-co-3-acrylamidopropyl trimethylammonium chloride (APTAC)-co-tert-butylacrylamide), was conjugated to the surface of silica beads through surface-initiated atom transfer radical polymerization. Prepared copolymer- and copolymer brush-modified beads were characterized by CHN elemental analysis, X-ray photoelectron spectroscopy, gel permeation chromatography, and observation of phase transition profiles. Phase transition profiles of the prepared copolymer indicated that 5 mol % APTAC is suitable for enabling thermally modulated property changes in the copolymer. Chromatographic elution behaviors of adenosine nucleotides and proteins were observed using prepared beads as chromatography matrices. Higher retention time of adenosine nucleotides and strong protein adsorption behavior were observed compared with those on beads with tertiary amine groups, because of the strong basic properties. Therefore, copolymer brush modified beads will be useful as thermoresponsive ion-exchange chromatographic matrices.
| 元の言語 | English |
|---|---|
| ページ（範囲） | 1031-1043 |
| ページ数 | 13 |
| ジャーナル | Biomacromolecules |
| 巻 | 15 |
| 発行部数 | 3 |
| DOI | |
| 出版物ステータス | Published - 2014 3 10 |
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ASJC Scopus subject areas
- Bioengineering
- Materials Chemistry
- Polymers and Plastics
- Biomaterials
これを引用
Thermoresponsive copolymer brushes possessing quaternary amine groups for strong anion-exchange chromatographic matrices. / Nagase, Kenichi; Geven, Mike; Kimura, Saori; Kobayashi, Jun; Kikuchi, Akihiko; Akiyama, Yoshikatsu; Grijpma, Dirk W.; Kanazawa, Hideko; Okano, Teruo.
：: Biomacromolecules, 巻 15, 番号 3, 10.03.2014, p. 1031-1043.研究成果: Article
}
TY - JOUR
T1 - Thermoresponsive copolymer brushes possessing quaternary amine groups for strong anion-exchange chromatographic matrices
AU - Nagase, Kenichi
AU - Geven, Mike
AU - Kimura, Saori
AU - Kobayashi, Jun
AU - Kikuchi, Akihiko
AU - Akiyama, Yoshikatsu
AU - Grijpma, Dirk W.
AU - Kanazawa, Hideko
AU - Okano, Teruo
PY - 2014/3/10
Y1 - 2014/3/10
N2 - A thermoresponsive copolymer incorporating a quaternary amine group, poly(N-isopropylacrylamide-co-3-acrylamidopropyl trimethylammonium chloride (APTAC)-co-tert-butylacrylamide), was conjugated to the surface of silica beads through surface-initiated atom transfer radical polymerization. Prepared copolymer- and copolymer brush-modified beads were characterized by CHN elemental analysis, X-ray photoelectron spectroscopy, gel permeation chromatography, and observation of phase transition profiles. Phase transition profiles of the prepared copolymer indicated that 5 mol % APTAC is suitable for enabling thermally modulated property changes in the copolymer. Chromatographic elution behaviors of adenosine nucleotides and proteins were observed using prepared beads as chromatography matrices. Higher retention time of adenosine nucleotides and strong protein adsorption behavior were observed compared with those on beads with tertiary amine groups, because of the strong basic properties. Therefore, copolymer brush modified beads will be useful as thermoresponsive ion-exchange chromatographic matrices.
AB - A thermoresponsive copolymer incorporating a quaternary amine group, poly(N-isopropylacrylamide-co-3-acrylamidopropyl trimethylammonium chloride (APTAC)-co-tert-butylacrylamide), was conjugated to the surface of silica beads through surface-initiated atom transfer radical polymerization. Prepared copolymer- and copolymer brush-modified beads were characterized by CHN elemental analysis, X-ray photoelectron spectroscopy, gel permeation chromatography, and observation of phase transition profiles. Phase transition profiles of the prepared copolymer indicated that 5 mol % APTAC is suitable for enabling thermally modulated property changes in the copolymer. Chromatographic elution behaviors of adenosine nucleotides and proteins were observed using prepared beads as chromatography matrices. Higher retention time of adenosine nucleotides and strong protein adsorption behavior were observed compared with those on beads with tertiary amine groups, because of the strong basic properties. Therefore, copolymer brush modified beads will be useful as thermoresponsive ion-exchange chromatographic matrices.
UR - http://www.scopus.com/inward/record.url?scp=84896767501&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84896767501&partnerID=8YFLogxK
U2 - 10.1021/bm401918a
DO - 10.1021/bm401918a
M3 - Article
C2 - 24467304
AN - SCOPUS:84896767501
VL - 15
SP - 1031
EP - 1043
JO - Biomacromolecules
JF - Biomacromolecules
SN - 1525-7797
IS - 3
ER -