Tight-binding inhibitory sequences against pp60(c-src) identified using a random 15-amino-acid peptide library

Toru Nishi, Raymond J.A. Budde, John S. McMurray, Nihal U. Obeyesekere, Naueen Safdar, Victor A. Levin, Hideyuki Saya

研究成果: Article査読

41 被引用数 (Scopus)

抄録

A bacteriophage peptide library containing a random 15-amino-acid insert was screened for identification of peptide sequence(s) that bind pp60(c-src). Sequencing the random insert from more than 100 virions indicated that more than 60% of the phage virions that bound to this enzyme contained a GXXG sequence motif in which X was frequently a hydrophobic residue. The GXXG sequence was often repeated as GXXGXXG. Two nonameric peptides were synthesized to determine whether or not the peptide inhibits pp60(c-src) tyrosine kinase activity and the importance of the glycine residues within this sequence. The peptide containing glycine had a K(i) of 24 μM, whereas replacing the glycines with proline increased the K(i) value to 3.1 mM.

本文言語English
ページ(範囲)237-240
ページ数4
ジャーナルFEBS Letters
399
3
DOI
出版ステータスPublished - 1996 12月 16
外部発表はい

ASJC Scopus subject areas

  • 生物理学
  • 構造生物学
  • 生化学
  • 分子生物学
  • 遺伝学
  • 細胞生物学

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