@article{88a3fb405343492a882f1f84e3ab8645,
title = "Time-lapse monitoring of TLR2 ligand internalization with newly developed fluorescent probes",
abstract = "As a mammalian toll-like receptor family member protein, TLR2 recognizes lipoproteins from bacteria and modulates the immune response by inducing the expression of various cytokines. We have developed fluorescence-labeled TLR2 ligands with either hydrophilic or hydrophobic fluorescence groups. The labeled ligands maintained the inflammatory IL-6 induction activity and enabled us to observe the internalization and colocalization of the TLR2 ligands using live-cell imaging. The time-lapse monitoring in the live-cell imaging of the fluorescence-labeled TLR2 ligand showed that TLR2/CD14 expression in the host cells enhanced the internalization of TLR2 ligand molecules.",
author = "Yohei Arai and Kouhei Yokoyama and Yuki Kawahara and Qi Feng and Ippei Ohta and Atsushi Shimoyama and Shinsuke Inuki and Koichi Fukase and Kazuya Kabayama and Yukari Fujimoto",
note = "Funding Information: This work was supported by Grants-in-Aid for Scientific Research, KAKENHI (Y. F.; JP26102732, JP26282211, JP17H02207, JP17H05800 and JP16H01162; K. F.; JP26882036, JP23241074, JP15H05836, and JP16H01885) by MEXT-Supported Program (JSPS) for the Strategic Research Foundation at Private Universities (K. K.; S1411010), by NEXT-program from JSPS (Y. F.; LR025), Mizutani Foundation for Glycoscience (Y. F.), Mishima Kaiun Memorial Foundation (Y. F.) and Takeda Science Foundation (Y. F.). Publisher Copyright: {\textcopyright} 2018 The Royal Society of Chemistry.",
year = "2018",
doi = "10.1039/c7ob03205f",
language = "English",
volume = "16",
pages = "3824--3830",
journal = "Organic and Biomolecular Chemistry",
issn = "1477-0520",
publisher = "Royal Society of Chemistry",
number = "20",
}