Time-lapse monitoring of TLR2 ligand internalization with newly developed fluorescent probes

Yohei Arai, Kouhei Yokoyama, Yuki Kawahara, Qi Feng, Ippei Ohta, Atsushi Shimoyama, Shinsuke Inuki, Koichi Fukase, Kazuya Kabayama, Yukari Fujimoto

研究成果: Article査読

2 被引用数 (Scopus)

抄録

As a mammalian toll-like receptor family member protein, TLR2 recognizes lipoproteins from bacteria and modulates the immune response by inducing the expression of various cytokines. We have developed fluorescence-labeled TLR2 ligands with either hydrophilic or hydrophobic fluorescence groups. The labeled ligands maintained the inflammatory IL-6 induction activity and enabled us to observe the internalization and colocalization of the TLR2 ligands using live-cell imaging. The time-lapse monitoring in the live-cell imaging of the fluorescence-labeled TLR2 ligand showed that TLR2/CD14 expression in the host cells enhanced the internalization of TLR2 ligand molecules.

本文言語English
ページ(範囲)3824-3830
ページ数7
ジャーナルOrganic and Biomolecular Chemistry
16
20
DOI
出版ステータスPublished - 2018

ASJC Scopus subject areas

  • 生化学
  • 物理化学および理論化学
  • 有機化学

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