Time resolved DNA occupancy dynamics during the respiratory oscillation uncover a global reset point in the yeast growth program

Cornelia Amariei, Rainer Machné, Viktor Stolc, Tomoyoshi Soga, Masaru Tomita, Douglas B. Murray

研究成果: Article査読

8 被引用数 (Scopus)

抄録

The structural dynamics of chromatin have been implicated in the regulation of fundamental eukaryotic processes, such as DNA transcription, replication and repair. Although previous studies have revealed that the chromatin landscape, nucleosome remodeling and histone modification events are intimately tied into cellular energetics and redox state, few studies undertake defined time-resolved measurements of these state variables. Here, we use metabolically synchronous, continuously-grown yeast cultures to measure DNA occupancy and track global patterns with respect to the metabolic state of the culture. Combined with transcriptome analyses and ChIP-qPCR experiments, these paint an intriguing picture where genome-wide nucleosome focusing occurs during the recovery of energy charge, followed by clearance of the promoter regions and global transcriptional slow-down, thus indicating a nucleosome-mediated “reset point” for the cycle. The reset begins at the end of the catabolic and stress-response transcriptional programs and ends prior to the start of the anabolic and cell-growth transcriptional program, and the histones on genes from both the catabolic and anabolic superclusters are deacetylated.

本文言語English
ページ(範囲)279-288
ページ数10
ジャーナルMicrobial Cell
1
9
DOI
出版ステータスPublished - 2014 9

ASJC Scopus subject areas

  • 免疫学および微生物学(その他)
  • 生化学、遺伝学、分子生物学(その他)

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