Tools for Genome Synthesis

Mitsuhiro Itaya

研究成果: Chapter

2 引用 (Scopus)

抄録

The advent of genome synthesis in the 21st century opened up various approaches on cloning whole bacterial genomes. In particular, relevant technologies starting from chemically synthesized oligomers should enable genome reorganization not limited to existing genomes but expanded to any genomes with novel sequences designed even from scratch. Those cutting-edge technologies sooner or later will replace template-dependent gene cloning technologies, albeit technical difficulties regarding efficiency and cost preclude general use at present. Meanwhile, rationally designed genomes require thorough knowledge of existing genomes in retrospect. Certain unnoticed rules underlying functional genomes might be found through various kinds and levels of studies.This chapter recaps the presently available tools and relevant ideas towards production of giant DNA molecules equivalent to or potentially larger-sized bacterial genomes, particularly focusing on our host, Bacillus subtilis 168. This chapter also describes the future perspectives of deep understanding on cellular systems innovated by and viewed from the genome synthesis technology. © 2013

元の言語English
ホスト出版物のタイトルSynthetic Biology
出版者Elsevier Inc.
ページ225-242
ページ数18
ISBN(印刷物)9780123944306
DOI
出版物ステータスPublished - 2013

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Genes
Genome
Technology
Bacterial Genomes
Organism Cloning
Cloning
Bacillus subtilis
Bacilli
Oligomers
Costs and Cost Analysis
DNA
Molecules
Costs

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

これを引用

Itaya, M. (2013). Tools for Genome Synthesis. : Synthetic Biology (pp. 225-242). Elsevier Inc.. https://doi.org/10.1016/B978-0-12-394430-6.00012-1

Tools for Genome Synthesis. / Itaya, Mitsuhiro.

Synthetic Biology. Elsevier Inc., 2013. p. 225-242.

研究成果: Chapter

Itaya, M 2013, Tools for Genome Synthesis. : Synthetic Biology. Elsevier Inc., pp. 225-242. https://doi.org/10.1016/B978-0-12-394430-6.00012-1
Itaya M. Tools for Genome Synthesis. : Synthetic Biology. Elsevier Inc. 2013. p. 225-242 https://doi.org/10.1016/B978-0-12-394430-6.00012-1
Itaya, Mitsuhiro. / Tools for Genome Synthesis. Synthetic Biology. Elsevier Inc., 2013. pp. 225-242
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