Top3β is an RNA topoisomerase that works with fragile X syndrome protein to promote synapse formation

Dongyi Xu, Weiping Shen, Rong Guo, Yutong Xue, Wei Peng, Jian Sima, Jay Yang, Alexei Sharov, Subramanya Srikantan, Jiandong Yang, David Fox, Yong Qian, Jennifer L. Martindale, Yulan Piao, James Machamer, Samit R. Joshi, Subhasis Mohanty, Albert C. Shaw, Thomas E. Lloyd, Grant W. BrownMinoru S.H. Ko, Myriam Gorospe, Sige Zou, Weidong Wang

研究成果: Article査読

73 被引用数 (Scopus)

抄録

Topoisomerases are crucial for solving DNA topological problems, but they have not been linked to RNA metabolism. Here we show that human topoisomerase 3β (Top3β) is an RNA topoisomerase that biochemically and genetically interacts with FMRP, a protein that is deficient in fragile X syndrome and is known to regulate the translation of mRNAs that are important for neuronal function, abnormalities of which are linked to autism. Notably, the FMRP-Top3β interaction is abolished by a disease-associated mutation of FMRP, suggesting that Top3β may contribute to the pathogenesis of mental disorders. Top3β binds multiple mRNAs encoded by genes with neuronal functions linked to schizophrenia and autism. Expression of one such gene, that encoding protein tyrosine kinase 2 (ptk2, also known as focal adhesion kinase or FAK), is reduced in the neuromuscular junctions of Top3β mutant flies. Synapse formation is defective in Top3β mutant flies and mice, as well as in FMRP mutant flies and mice. Our findings suggest that Top3β acts as an RNA topoisomerase and works with FMRP to promote the expression of mRNAs that are crucial for neurodevelopment and mental health.

本文言語English
ページ(範囲)1238-1247
ページ数10
ジャーナルNature Neuroscience
16
9
DOI
出版ステータスPublished - 2013 9

ASJC Scopus subject areas

  • Neuroscience(all)

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