Towards an understanding of channelrhodopsin function: Simulations lead to novel insights of the channel mechanism

Hiroshi C. Watanabe, Kai Welke, Daniel J. Sindhikara, Peter Hegemann, Marcus Elstner

研究成果: Article

48 引用 (Scopus)

抜粋

Channelrhodopsins (ChRs) are light-gated cation channels that mediate ion transport across membranes in microalgae (vectorial catalysis). ChRs gain increasing attention as useful tools for the analysis of neural networks in tissues and living animals (optogenetics). In fact, various mutagenesis approaches have realized practical applications with high reliability by enhancement of the expression level, channel kinetics control, and color tuning. Furthermore, the recently published x-ray structure has provided valuable information for further atomistic studies and engineering ChRs for a wider application. The present study is a computational attempt to describe the functional mechanism at the atomic level based on the x-ray structure. We present several structural characteristics that are highly involved in ion channel gating and ion transport, including (1) water distribution, (2) cation binding sites, (3) intrahelical hydrogen bond, (4) DC gate, and (5) active site.

元の言語English
ページ(範囲)1795-1814
ページ数20
ジャーナルJournal of Molecular Biology
425
発行部数10
DOI
出版物ステータスPublished - 2013 5 27
外部発表Yes

ASJC Scopus subject areas

  • Structural Biology
  • Molecular Biology

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