Transcellular transport of genistein, a soybean-derived Isoflavone, across human colon carcinoma cell line

Masataka Oitate, Rie Nakaki, Noriko Koyabu, Hitomi Takanaga, Hirotami Matsuo, Hisakazu Ohtani, Yasufumi Sawada

研究成果: Article

29 引用 (Scopus)

抄録

Genistein, a soybean-derived isoflavone, is thought to have an anticarcinogenic action, but little is known about the cellular mechanisms of its intestinal absorption. This study was designed to investigate the absorption mechanisms of genistein using human colon carcinoma cell line, Caco-2 cells. The apical-to-basolateral transcellular transport of genistein across a Caco-2 cell monolayer was significantly greater than that in the opposite direction. An uptake experiment revealed that cellular uptake of genistein by Caco-2 cells was concentrative. The transcellular transport of genistein was saturable and temperature-dependent, and was inhibited by other flavonoids such as rutin, quercetin, (+)-catechin and (-)-epicatechin. These results suggest that genistein is transported across Caco-2 cells by a carrier-mediated system, located on the apical membrane.

元の言語English
ページ(範囲)23-29
ページ数7
ジャーナルBiopharmaceutics and Drug Disposition
22
発行部数1
DOI
出版物ステータスPublished - 2001
外部発表Yes

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Transcytosis
Isoflavones
Genistein
Soybeans
Colon
Caco-2 Cells
Carcinoma
Cell Line
Catechin
Rutin
Quercetin
Intestinal Absorption
Flavonoids
Temperature
Membranes

ASJC Scopus subject areas

  • Pharmacology (medical)
  • Pharmacology, Toxicology and Pharmaceutics(all)

これを引用

Transcellular transport of genistein, a soybean-derived Isoflavone, across human colon carcinoma cell line. / Oitate, Masataka; Nakaki, Rie; Koyabu, Noriko; Takanaga, Hitomi; Matsuo, Hirotami; Ohtani, Hisakazu; Sawada, Yasufumi.

:: Biopharmaceutics and Drug Disposition, 巻 22, 番号 1, 2001, p. 23-29.

研究成果: Article

Oitate, Masataka ; Nakaki, Rie ; Koyabu, Noriko ; Takanaga, Hitomi ; Matsuo, Hirotami ; Ohtani, Hisakazu ; Sawada, Yasufumi. / Transcellular transport of genistein, a soybean-derived Isoflavone, across human colon carcinoma cell line. :: Biopharmaceutics and Drug Disposition. 2001 ; 巻 22, 番号 1. pp. 23-29.
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AU - Nakaki, Rie

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AU - Takanaga, Hitomi

AU - Matsuo, Hirotami

AU - Ohtani, Hisakazu

AU - Sawada, Yasufumi

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