Transcriptome Analysis of Adrenocortical Cells in Health and Disease

研究成果: Chapter

抄録

The transcriptome is the complete set of transcripts in a specific type of cell or tissue. Generally, the goal of transcriptome analysis is to identify genes differentially expressed among different conditions, leading to a new understanding of the genes or pathways associated with the conditions. Transcriptome analysis requires an appropriate statistical method with a multiple comparison test to interpret global changes in the expression of thousands of genes. This chapter overviews and describes recent progress in the transcriptome analysis of adrenocortical cells. Strategies for determining transcriptomes and data processing to interpret analysis have been discussed. Representative studies have been introduced: an adrenocortical cell line with small interference RNA-knockdown experiment revealed steroidogenic factor-1 functions as a “regulone” that orchestrate tissue-specific steroidogenic regulation and housekeeping metabolic regulation and a mouse model for human lipoid congenital adrenal hyperplasia indicated a reciprocal interaction between steroidogenic cells and adjacent macrophages both fulfilled with cholesterol ester droplets. Analysis of global transcript profiling will be an essential tool for future research on the nature of adrenocortical cells.

本文言語English
ホスト出版物のタイトルCellular Endocrinology in Health and Disease, Second Edition
出版社Elsevier
ページ349-374
ページ数26
ISBN(電子版)9780128198018
DOI
出版ステータスPublished - 2021 1月 1

ASJC Scopus subject areas

  • 農業および生物科学(全般)
  • 生化学、遺伝学、分子生物学(全般)

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